Effects of Caffeine on Olfactory Learning in Crickets
Effects of Caffeine on Olfactory Learning in Crickets
复制标题
咖啡因对蟋蟀嗅觉学习的影响
作者：
S. Sugimachi;Y. Matsumoto;M. Mizunami;J. Okada
咖啡因是一种植物衍生的生物碱，通常被称为中枢神经系统（CNS）兴奋剂。为了研究咖啡因对昆虫高级中枢神经系统功能的影响，我们使用了一个食欲嗅觉学习模式的蟋蟀双斑。蟋蟀可以在重复训练后形成重要的长期记忆（LTM），在此期间，它们将条件刺激（CS：气味）与非条件刺激（US：奖励）联系起来。在广泛的咖啡因剂量范围内（1.6 µg/kg至39 mg/kg），仅在单次试验条件下，给予血淋巴注射咖啡因即可建立LTM。我们研究了这种增强嗅觉学习表现的生理机制，重点是咖啡因的三个主要生理作用：1）抑制磷酸二酯酶（PDE），2）激动ryanodine受体，3）拮抗腺苷受体。与这些作用相关的药物的应用对LTM形成产生显著影响。这些结果表明，外部应用咖啡因通过多种细胞机制增强昆虫嗅觉学习中的LTM形成。
Caffeine is a plant-derived alkaloid that is generally known as a central nervous system (CNS) stimulant. In order to examine the effects of caffeine on higher CNS functions in insects, we used an appetitive olfactory learning paradigm for the cricket Gryllus bimaculatus. Crickets can form significant long-term memories (LTMs) after repetitive training sessions, during which they associate a conditioned stimulus (CS: odor) with an unconditioned stimulus (US: reward). Administration of hemolymphal injections of caffeine established LTM after only single-trial conditioning over a wide range of caffeine dosages (1.6 µµg/kg to 39 mg/kg). We investigated the physiological mechanisms underlying this enhancement of olfactory learning performance pharmacologically, focusing on three major physiological roles of caffeine: 1) inhibition of phosphodiesterase (PDE), 2) agonism of ryanodine receptors, and 3) antagonism of adenosine receptors. Application of drugs relevant to these actions resulted in significant effects on LTM formation. These results suggest that externally applied caffeine enhances LTM formation in insect olfactory learning via multiple cellular mechanisms.
影响因子：
21.1
作者：
B. Fredholm;K. Bättig;J. Holmén;A. Nehlig;E. Zvartau
通讯作者：
B. Fredholm;K. Bättig;J. Holmén;A. Nehlig;E. Zvartau
影响因子：
6.1
作者：
Rivera-Oliver, Marla;Diaz-Rios, Manuel
通讯作者：
Diaz-Rios, Manuel