Monoclonal antibodies raised to human cells - specificity for pig leukocytes
Monoclonal antibodies raised to human cells - specificity for pig leukocytes
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DOI：
10.1016/s0165-2427(01)00285-9
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发表时间：
2001-07-20
影响因子：
1.8
通讯作者：
Ledbetter, JA
中科院分区：
文献类型：
--
作者：
Haverson, K;Bailey, M;Ledbetter, JA
目前的猪CD研讨会共包括27种针对人靶点的单克隆抗体。其中14种已在之前的研讨会上进行了测试，并报告为交叉反应性，另有13种在人类白细胞分化抗原研讨会VI（HLDA VI）期间和/或由捐赠者（提交这些mAb供研讨会团体验证的商业公司）报告为交叉反应性。在27种抗体中，3种先前报告对猪细胞具有反应性的抗体在初步试验后因缺乏反应性而从车间中淘汰。9种抗体虽然最初呈阳性，但在讲习班期间得出的结果不一致。我们发现15种抗体的反应性一致。然而，对于这些抗体中的三种，靶分子在猪和人细胞上的细胞分布显示出不同。这些发现对于这些抗体作为猪的研究工具的有用性具有重要意义。(C)2001爱思唯尔科技有限公司。保留所有权利。
A total of 27 monoclonal antibodies raised to human targets were included in the present Pig CD workshop. 14 of these had been tested in previous workshops and had been reported as crossreactive, a further 13 had been reported as cross-reactive during the Human Leukocyte Differentiation Antigens Workshop VI (HLDA VI) and/or by the donor (a commercial company submitting these mAb for validation by the workshop community). Of the 27 antibodies, three antibodies with previously reported reactivity for pig cells were eliminated from the workshop following preliminary tests due to lack of reactivity. Nine antibodies, although initially positive, gave inconsistent results during the course of the workshop. We found consistent reactivity for 15 antibodies. However, the cellular distribution of the target molecules on pig and human cells was shown to be different for three of these antibodies. These findings have important implications for the usefulness of these antibodies as research tools in the pig. (C) 2001 Elsevier Science B.V. All rights reserved.