Human papillomavirus type 11 recombinant L1 capsomeres induce virus-neutralizing antibodies
Human papillomavirus type 11 recombinant L1 capsomeres induce virus-neutralizing antibodies
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DOI：
10.1128/jvi.72.7.6151-6154.1998
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发表时间：
1998-07-01
影响因子：
5.4
通讯作者：
Garcea, RL
中科院分区：
文献类型：
--
作者：
Rose, RC;White, WI;Garcea, RL
人乳头瘤病毒11型(HPV-11)L1主要衣壳蛋白可被胰酶消化以产生保留HPV基因限制性衣壳抗原性的重组衣壳(M.Li，T.P.Gripe，P.A.Estes，M.K.Lyon，R.C.Rose，和R.L.Garcea，J.Virol。71：2988-2995,1997)，在本研究中，先前被描述为识别两个不同的HPV-11衣壳中和抗原域(S.W.Ludmerer，D.Benincasa和G.E.Mark III，J.Virol，70：4791-4794,1996)的HPV-11病毒粒子中和单抗H11.F1和H11.H3在酶联免疫吸附试验(ELISA)中被发现与胰酶产生的衣壳高度免疫反应。然后，在体外感染性试验中测试了这些胶囊抗血清，发现它们能够中和HPV-11病毒粒子。在本试验中，HPV-11衣壳多克隆抗血清的中和效价为10(-5)到10(-6)，与先前针对完整的HPV-11 VLP(R.C.Rose，R.C.Reichman和W.Bonnez，J.Gen，Virol)获得的病毒粒子中和抗血清相当。75：2075-2079,1994)，这些结果表明，高度免疫原性、基因限制性的HPV衣壳中和抗原域完全包含在衣壳内。因此，胶囊可能是预防HPV疾病的可行疫苗候选。
The human papillomavirus type 11 (HPV-11) L1 major capsid protein can be trypsinized to generate recombinant capsomeres that retain HPV genotype-restricted capsid antigenicity (M. Li, T. P. Gripe, P. A. Estes, M. K. Lyon, R. C. Rose, and R. L. Garcea, J. Virol. 71:2988-2995, 1997), In the present study, HPV-11 virion-neutralizing monoclonal antibodies H11.F1 and H11.H3, previously characterized as recognizing two distinct HPV-11 capsid-neutralizing antigenic domains (S. W. Ludmerer, D. Benincasa, and G. E. Mark III, J. Virol, 70:4791-4794, 1996), were each found to be highly immunoreactive with trypsin-generated capsomeres in an enzyme-linked immunosorbent assay (ELISA), Capsomeres were used to generate high-titer polyclonal immune sera that demonstrated HPV genotype-restricted reactivity by ELISA, The capsomere antisera were then tested in an in vitro infectivity assay and found to neutralize HPV-11 virions. In this assay, HPV-11 capsomere polyclonal antisera exhibited neutralization titers (10(-5) to 10(-6)) comparable to those obtained with a virion-neutralizing antiserum raised previously against intact HPV-11 VLPs (R. C. Rose, R. C. Reichman, and W. Bonnez, J. Gen, Virol. 75:2075-2079, 1994), These results indicate that highly immunogenic, genotype-restricted HPV capsid-neutralizing antigenic domains are contained entirely within capsomeres. Thus, capsomeres may be viable vaccine candidates for the prevention of HPV disease.