Dynamic states of cervical epithelia during pregnancy and epithelial barrier disruption.
Dynamic states of cervical epithelia during pregnancy and epithelial barrier disruption.
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DOI：
10.1016/j.isci.2023.105953
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发表时间：
2023-02-17
期刊：
影响因子：
5.8
通讯作者：
Mahendroo, Mala
中科院分区：
文献类型：
--
作者：
Cooley, Anne;Madhukaran, ShanmugaPriyaa;Stroebele, Elizabeth;Caraballo, Mariano Colon;Wang, Lei;Akgul, Yucel;Hon, Gary C.;Mahendroo, Mala
子宫颈上皮经历增殖、分化和功能的变化，这些变化对确保生育能力和维持妊娠至关重要。在这里，我们使用单细胞转录组和空间分析来识别非妊娠、妊娠和分娩小鼠的宫颈上皮亚型。我们确定异质亚群的上皮细胞显示空间和时间特异性。值得注意的是，在怀孕期间，两种杯状细胞亚型存在于最管腔层中，其中一种杯状细胞群在怀孕期间比另一种杯状细胞群更早地扩增。杯状种群表达新的保护因子和不同的粘膜网络。宫颈上皮屏障破坏模型中的单细胞分析表明，基底细胞过早增殖先于杯状细胞扩张，粘膜完整性降低。这些数据表明，宫颈上皮如何经历持续重塑，以维持动态的稳态在怀孕和分娩，并提供了一个框架，以了解扰动上皮健康，增加早产的风险。非妊娠期、妊娠期和分娩期小鼠宫颈单细胞分析宫颈上皮细胞亚群显示空间和时间特异性杯状细胞是妊娠期和分娩期的主要亚型分娩期间，转录重置扩展非妊娠上皮细胞群妊娠;生物科学;遗传学;基因组学
The cervical epithelium undergoes changes in proliferation, differentiation, and function that are critical to ensure fertility and maintain pregnancy. Here, we identify cervical epithelial subtypes in non-pregnant, pregnant, and in labor mice using single-cell transcriptome and spatial analysis. We identify heterogeneous subpopulations of epithelia displaying spatial and temporal specificity. Notably in pregnancy, two goblet cell subtypes are present in the most luminal layers with one goblet population expanding earlier in pregnancy than the other goblet population. The goblet populations express novel protective factors and distinct mucosal networks. Single-cell analysis in a model of cervical epithelial barrier disruption indicates untimely basal cell proliferation precedes the expansion of goblet cells with diminished mucosal integrity. These data demonstrate how the cervical epithelium undergoes continuous remodeling to maintain dynamic states of homeostasis in pregnancy and labor, and provide a framework to understand perturbations in epithelial health that increase the risk of premature birth. Mouse cervix single-cell analysis in non-pregnancy, pregnancy, and in labor Cervix epithelia subpopulations display spatial and temporal specificity Goblet cells are the dominant subtype in pregnancy and in labor During labor, transcriptional reset expands non-pregnant epithelial populations Pregnancy; Biological sciences; Genetics; Genomics
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影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
3.8
作者：
Florova V;Romero R;Tarca AL;Galaz J;Motomura K;Ahmad MM;Hsu CD;Hsu R;Tong A;Ravel J;Theis KR;Gomez-Lopez N
通讯作者：
Gomez-Lopez N
影响因子：
16.6
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Chan D;Bennett PR;Lee YS;Kundu S;Teoh TG;Adan M;Ahmed S;Brown RG;David AL;Lewis HV;Gimeno-Molina B;Norman JE;Stock SJ;Terzidou V;Kropf P;Botto M;MacIntyre DA;Sykes L
通讯作者：
Sykes L
影响因子：
4
作者：
GALAND, P;LEROY, F;CHRETIEN, J
通讯作者：
CHRETIEN, J
影响因子：
6.4
作者：
Burcham, Lindsey R.;Bath, Jade R.;Werlang, Caroline A.;Lyon, Laurie M.;Liu, Naoko;Evans, Christopher;Ribbeck, Katharina;Doran, Kelly S.
通讯作者：
Doran, Kelly S.