A NOVEL DISULFIDE-LINKED HETERODIMER ON PRE-T-CELLS CONSISTS OF THE T-CELL RECEPTOR-BETA CHAIN AND A 33 KD GLYCOPROTEIN
A NOVEL DISULFIDE-LINKED HETERODIMER ON PRE-T-CELLS CONSISTS OF THE T-CELL RECEPTOR-BETA CHAIN AND A 33 KD GLYCOPROTEIN
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DOI：
10.1016/0092-8674(93)80070-u
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发表时间：
1993-10-22
期刊：
影响因子：
64.5
通讯作者：
VONBOEHMER, H
中科院分区：
文献类型：
--
作者：
GROETTRUP, M;UNGEWISS, K;VONBOEHMER, H
我们描述了一种新的信号转导蛋白复合体，它由T细胞受体(TCR)β链组成，该链二硫键连接到33kd的糖蛋白上，并与T前细胞系SCB.29表面的CD3复合体的蛋白质非共价结合。这个33kd的糖蛋白，暂时命名为gp33，既不代表已知的TCR链，也没有被先前检测到，因为它的表面碘化标记很差。这种糖蛋白在成熟T细胞系的表面缺失。从TCRα缺陷小鼠的表面碘标记的大胸腺细胞中可以免疫沉淀出还原前后分子质量相同的TCRβ复合体。新的gp33-TCRβ复合体可能完全或部分地控制了TCRβ蛋白对早期T细胞的发育。
We describe a novel signal-transducing protein complex, which consists of the T cell receptor (TCR) beta chain that is disulfide linked to a 33 kd glycoprotein and non-covalently associated with proteins of the CD3 complex on the surface of the pre-T cell line SCB.29. This 33 kd glycoprotein, provisionally designated gp33, represents neither of the known TCR chains and has escaped previous detection because it labels poorly by surface iodination. This glycoprotein is absent from the surface of mature T cell lines. A TCR beta complex with identical molecular masses before and after reduction can be immunoprecipitated from surface-iodinated large thymocytes of TCR alpha-deficient mice. The novel gp33-TCR beta complex may be entirely or partly responsible for control of early T cell development exerted by the TCR beta protein.