Structural observation of the primary isomerization in vision with femtosecond-stimulated Raman
Structural observation of the primary isomerization in vision with femtosecond-stimulated Raman
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DOI：
10.1126/science.1118379
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发表时间：
2005-11-11
期刊：
影响因子：
56.9
通讯作者：
Mathies, RA
中科院分区：
文献类型：
--
作者：
Kukura, P;McCamant, DW;Mathies, RA
引发视觉的主要事件是光诱导的视色素视紫红质中的11-顺式至全反式的视网膜异构化。尽管使用化学反应动力学的传统工具进行了数十年的研究，但导致光产物产生的原子运动的时间和性质仍然未知。我们使用飞秒受激拉曼光谱获得的时间分辨振动光谱的分子结构形成的沿着反应坐标。光子吸收后从200飞秒到1皮秒的振动特征的光谱演变揭示了激活该受体的视网膜主干几何变化的时间顺序。
The primary event that initiates vision is the light-induced 11-cis to all-trans isomerization of retinal in the visual pigment rhodopsin. Despite decades of study with the traditional tools of chemical reaction dynamics, both the timing and nature of the atomic motions that lead to photoproduct production remain unknown. We used femtosecond-stimulated Raman spectroscopy to obtain time-resolved vibrational spectra of the molecular structures formed along the reaction coordinate. The spectral evolution of the vibrational features from 200 femtoseconds to 1 picosecond after photon absorption reveals the temporal sequencing of the geometric changes in the retinal backbone that activate this receptor.