Antibiotic susceptibilities and resistance genes of Ureaplasma parvum isolated in South Africa
Antibiotic susceptibilities and resistance genes of Ureaplasma parvum isolated in South Africa
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DOI：
10.1093/jac/dks314
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发表时间：
2012-12-01
影响因子：
5.2
通讯作者：
Chalkley, L. J.
中科院分区：
文献类型：
--
作者：
Govender, S.;Gqunta, K.;Chalkley, L. J.
关于南非微小脲原体的抗菌药物敏感性和耐药基因的信息有限。本研究的目的是检测和表征抗稻瘟病基因。15 U.采用肉汤微量稀释法（四环素、强力霉素、氧氟沙星、红霉素、阿奇霉素和交沙霉素）研究细小病毒分离株。对以下靶区域进行基因分析：泰特（M）; gyrA、gyrB、parC和帕雷; gyrA（A）、gyrB（B）、gyrC（C）和gyrE（E）; msr（A）、msr（B）、msr（C）和msr（D）; 23 S rRNA操纵子;以及L4和L22核糖体蛋白。细小病毒分离株对所测试的抗生素完全敏感。5株对四环素耐药（MIC为16256 mg/L），1株对氧氟沙星耐药（MIC为128 mg/L），4株对大环内酯类耐药（MIC为128 mg/L），2株对四环素和红霉素双重耐药。发现5个四环素耐药菌株具有嵌合泰特（M）基因，其中一个菌株含有与先前描述的不同的特异性区域。在对红霉素（两种菌株）和红霉素阿奇霉素（一种菌株）耐药的三种菌株中观察到L22核糖体蛋白的突变。对红霉素和阿奇霉素耐药的菌株，其可能的耐药机制尚不清楚。产于南非。在氧氟沙星耐药菌株中发现了parC基因的一个点突变（Pro-57 Leu）和parE基因的两个新突变（Ile-73 Thr和密码子86处的甲硫氨酸插入）。这项研究加强了美国的适应性。小孢子虫产生抗性并获得、修饰和维持转座子定位的抗性基因。
There is only limited information on the antimicrobial susceptibilities and resistance genes of Ureaplasma parvum in South Africa. This study was designed to detect and characterize resistance genes in U. parvum.Fifteen U. parvum isolates were investigated employing the broth microdilution method (tetracycline, doxycycline, ofloxacin, erythromycin, azithromycin and josamycin). Gene analyses were performed on target regions of: tet(M); gyrA, gyrB, parC and parE; erm(A), erm(B), erm(C) and erm(E); msr(A), msr(B), msr(C) and msr(D); 23S rRNA operons; and L4 and L22 ribosomal proteins.Seven of the U. parvum isolates were fully susceptible to the antibiotics tested. Five strains exhibited resistance to tetracycline (MICs 16256 mg/L), one strain was resistant to ofloxacin (MIC 128 mg/L) and four strains were resistant to macrolides (MICs 128 mg/L); two strains showed dual resistance to tetracycline and erythromycin. The five tetracycline-resistant strains were found to have mosaic tet(M) genes, with one strain containing different specific regions to those previously described. Mutations in the L22 ribosomal protein were seen in three strains that were resistant to erythromycin (two strains) and erythromycinazithromycin (one strain). For a further strain that was resistant to erythromycin and azithromycin, possible mechanisms of resistance remained elusive.This is the first report of quinolone, erythromycin and azithromycin resistance development in U. parvum from South Africa. A point mutation in parC (Pro-57Leu) and two novel mutations in parE (Ile-73Thr and a methionine insertion at codon 86) were found in an ofloxacin-resistant strain. The study reinforces the adaptability of U. parvum to develop resistance and acquire, modify and maintain transposon-located resistance genes.