Identification of novel microRNAs in the Verticillium wilt-resistant upland cotton variety KV-1 by high-throughput sequencing.
Identification of novel microRNAs in the Verticillium wilt-resistant upland cotton variety KV-1 by high-throughput sequencing.
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作者：
He X;Sun Q;Jiang H;Zhu X;Mo J;Long L;Xiang L;Xie Y;Shi Y;Yuan Y;Cai Y
植物 microRNA (miRNA) 在发育、开花、植物生长、代谢和胁迫反应过程中的基因表达转录后调控中发挥着重要作用。黄萎病是植物维管束病害之一，由大丽花黄萎病引起，导致棉株变黄、萎蔫、倒伏、维管组织受损、死亡。陆地棉品种 KV-1 在多个水平上表现出对黄萎病的抗性。然而，关于抗性转录后调控的知识是有限的。本文以对黄萎病高度抗性的陆地棉品种 KV-1 幼苗为原料构建了两个新型小 RNA (sRNA) 文库，并接种了不同毒力菌株的 V991 和 D07038 大丽黄萎病 (V. dahliae)。通过 Illumina Solexa 系统对这两个文库进行测序后，鉴定出了 37 个新的 miRNA。根据序列同源性分析，预测了这些miRNA的潜在靶基因。由于新型 miRNA 和潜在靶标 mRNA 之间的序列不匹配不超过 3 个，我们预测了 24 种新型 miRNA 的 49 个靶标 mRNA。这些与基因相对应的靶mRNA被发现参与植物-病原体相互作用、内吞作用、丝裂原激活蛋白激酶(MAPK)信号通路以及异喹啉生物碱、萜类骨架、初级胆汁酸和次级代谢产物的生物合成。我们的结果表明，其中一些 miRNA 及其相关基因参与了黄萎病的抗性。陆地棉miRNA的鉴定和表征有助于进一步研究miRNA抗黄萎病的调控机制。本文的在线版本 (doi:10.1186/2193-1801-3-564) 包含补充材料，可供授权用户使用。
Plant microRNAs (miRNAs) play essential roles in the post-transcriptional regulation of gene expression during development, flowering, plant growth, metabolism, and stress responses. Verticillium wilt is one of the vascular disease in plants, which is caused by the Verticillium dahlia and leads to yellowing, wilting, lodging, damage to the vascular tissue, and death in cotton plants. Upland cotton varieties KV-1 have shown resistance to Verticillium wilt in multiple levels. However, the knowledge regarding the post-transcriptional regulation of the resistance is limited. Here two novel small RNA (sRNA) libraries were constructed from the seedlings of upland cotton variety KV-1, which is highly resistant to Verticillium wilts and inoculated with the V991 and D07038 Verticillium dahliae (V. dahliae) of different virulence strains. Thirty-seven novel miRNAs were identified after sequencing these two libraries by the Illumina Solexa system. According to sequence homology analysis, potential target genes of these miRNAs were predicted. With no more than three sequence mismatches between the novel miRNAs and the potential target mRNAs, we predicted 49 target mRNAs for 24 of the novel miRNAs. These target mRNAs corresponded to genes were found to be involved in plant–pathogen interactions, endocytosis, the mitogen-activated protein kinase (MAPK) signaling pathway, and the biosynthesis of isoquinoline alkaloid, terpenoid backbone, primary bile acid and secondary metabolites. Our results showed that some of these miRNAs and their relative gene are involved in resistance to Verticillium wilts. The identification and characterization of miRNAs from upland cotton could help further studies on the miRNA regulatory mechanisms of resistance to Verticillium wilt. The online version of this article (doi:10.1186/2193-1801-3-564) contains supplementary material, which is available to authorized users.
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影响因子：
12.3
作者：
Pang M;Woodward AW;Agarwal V;Guan X;Ha M;Ramachandran V;Chen X;Triplett BA;Stelly DM;Chen ZJ
通讯作者：
Chen ZJ
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
6.5
作者：
Barozai, Muhammad Younas Khan;Irfan, Muhammad;Riazuddin, Sheikh
通讯作者：
Riazuddin, Sheikh
影响因子：
4.4
作者：
Kwak PB;Wang QQ;Chen XS;Qiu CX;Yang ZM
通讯作者：
Yang ZM
影响因子：
2.7
作者：
Shi, R;Chiang, VL
通讯作者：
Chiang, VL