LncRNA AK001796 promotes cell proliferation via acting as a ceRNA of miR-150 in hepatocellular carcinoma.
LncRNA AK001796 promotes cell proliferation via acting as a ceRNA of miR-150 in hepatocellular carcinoma.
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DOI：
10.1590/1678-4685-gmb-2022-0277
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发表时间：
2023
影响因子：
2.1
通讯作者：
Yang, Qing
中科院分区：
文献类型：
--
作者：
Xu, Rui;He, Haitao;Wang, Yue;Peng, Qi;Mei, Ke;Liu, Yan;Yang, Qing
长链非编码RNA AK 001796最初在肺癌中被发现改变。近年来的研究表明，它可能参与肝细胞癌（HCC）的预后。然而，AK 001796的一般生物学作用及其在HCC中的潜在机制仍不清楚。在这里，我们证明了AK 001796在HCC组织和细胞系中的表达水平上调。沉默AK 001796抑制HCC细胞的增殖能力。通过双荧光素酶报告基因分析和功能丧失/获得研究，我们确定AK 001796可以结合miR-150，一种星星microRNA，促进HCC增殖。此外，据报道，生长因子受体结合蛋白2相关结合物1（GAB 1）是miR-150的靶基因。由于AK 001796是miR-150的诱饵并与GAB 1结合相同的miR-150推定位点，我们提出在AK 001796沉默细胞中抑制miR-150逆转了GAB 1的减少。随后，我们的研究结果表明，沉默AK 001796可以损害磷酸化ERK 1/2和磷酸化AKT。总之，我们的研究表明，AK 001796通过AK 001796/miR-150/GAB 1轴增强磷酸化ERK 1/2和磷酸化AKT促进HCC的增殖。这些结果为AK 001796积累HCC的关键作用提供了进一步的证据，并表明AK 001796可能在临床治疗中作为HCC生物标志物。
Long non-coding RNA AK001796 was initially identified altered in lung cancer. Recent research showed it could participate in the prognosis of hepatocellular carcinoma (HCC). However, the general biological role of AK001796 and its underlying mechanisms in HCC remain unclear. Here we demonstrated that the expression level of AK001796 in HCC tissues and cell lines was up-regulated. Silencing AK001796 suppressed the proliferation ability of HCC cells. Through dual luciferase reporter assays and loss/gain of functions studies, we identified that AK001796 could bind to miR-150, a star microRNA, promoting HCC proliferation. Furthermore, it was reported that growth factor receptor binding protein 2-associated binder 1 (GAB1) is a target gene of miR-150. Owing to AK001796 being a decoy for miR-150 and binding the same putative sites of miR-150 as GAB1, we presented that inhibition of miR-150 in AK001796 silencing cells reversed the reduction in GAB1. Subsequently, our findings demonstrated that silencing AK001796 can impair phospho-ERK1/2 and phospho-AKT. In conclusion, our investigation revealed that AK001796 promoted proliferation by enhancing phospho-ERK1/2 and phospho-AKT through AK001796/miR-150/GAB1 axis in HCC. These results provided further evidence for the critical roles of AK001796 accumulating HCC and suggested that AK001796 might act as an HCC biomarker in clinical treatment.
影响因子：
5.8
作者：
Liu B;Pan CF;Yao GL;Wei K;Xia Y;Chen YJ
通讯作者：
Chen YJ