Integrated RNA and miRNA sequencing analysis reveals a complex regulatory network of Magnolia sieboldii seed germination.
Integrated RNA and miRNA sequencing analysis reveals a complex regulatory network of Magnolia sieboldii seed germination.
复制标题
综合的RNA和miRNA测序分析揭示了一个复杂的Sieboldii种子发芽的调节网络。
DOI：
10.1038/s41598-021-90270-y
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发表时间：
2021-05-25
影响因子：
4.6
通讯作者：
Lu XJ
中科院分区：
文献类型：
--
作者：
Mei M;Wei J;Ai W;Zhang L;Lu XJ
三叶厚朴（Magnolia siboldii K. Koch）是中国厚朴科的一种落叶乔木，具有很高的观赏、药用和经济效益。三叶草种子在自然条件下发芽极其困难，从而阻碍了这一重要物种的培育和繁殖。由于缺乏基因组和转录组学资源，三叶草种子萌发的分子机制尚不清楚。在这里，我们整合了mRNA和miRNA测序来鉴定与三叶草发芽相关的基因和途径。通过单分子实时（SMRT）测序技术获得了包含158,083个高质量unigenes的完整全长转录组。我们共鉴定出13,877个基因在未发芽和发芽种子之间存在差异表达。这些基因主要参与植物激素信号转导和脂质、糖、氨基酸等多种代谢途径。我们的研究结果还确定了mirna与其靶基因之间复杂的调控网络。总之，我们提出了第一个三叶弓形虫的转录组，并提供了与种子萌发相关的关键基因和途径，以进一步表征。今后对三叶草种子萌发的分子基础的研究将有助于三叶草的遗传改良。
Magnolia sieboldii K. Koch (M. sieboldii) is a deciduous Chinese tree species of the Magnoliaceae family with high ornamental, medicinal, and economic benefits. The germination of M. sieboldii seeds under natural conditions is extremely difficult, thereby hindering the cultivation and breeding of this important species. The molecular mechanisms underlying M. sieboldii seed germination remain unclear due to the lack of genomic and transcriptomic resources. Here, we integrated both mRNA and miRNA sequencing to identify the genes and pathways related to M. sieboldii germination. A comprehensive full-length transcriptome containing 158,083 high-quality unigenes was obtained by single-molecule real-time (SMRT) sequencing technology. We identified a total of 13,877 genes that were differentially expressed between non-germinated and germinated seeds. These genes were mainly involved in plant hormone signal transduction and diverse metabolic pathways such as those involving lipids, sugars, and amino acids. Our results also identified a complex regulatory network between miRNAs and their target genes. Taken together, we present the first transcriptome of M. sieboldii and provide key genes and pathways associated with seed germination for further characterization. Future studies of the molecular basis of seed germination will facilitate the genetic improvement M. sieboldii.
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影响因子：
5.6
作者：
Galili G;Avin-Wittenberg T;Angelovici R;Fernie AR
通讯作者：
Fernie AR
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
7.4
作者：
Chauffour, Frederic;Bailly, Marlene;Marion-Poll, Annie
通讯作者：
Marion-Poll, Annie
影响因子：
7.4
作者：
Alonso-Peral, Maria M.;Li, Junyan;Millar, Anthony A.
通讯作者：
Millar, Anthony A.
DOI：
10.1073/pnas.1600558113
发表时间：
2016-04-12
影响因子：
11.1
作者：
Huo, Heqiang;Wei, Shouhui;Bradford, Kent J.
通讯作者：
Bradford, Kent J.