Family I84 protease inhibitors likely constitute a Mollusca-specific protein family functioning in host defense
Family I84 protease inhibitors likely constitute a Mollusca-specific protein family functioning in host defense
复制标题
DOI：
10.3354/dao03602
复制
发表时间：
2021-01-01
影响因子：
1.4
通讯作者：
Xue, Qinggang
中科院分区：
文献类型：
--
作者：
Lu, Jiali;Liu, Sheng;Xue, Qinggang
蛋白酶抑制剂是在所有生物体的许多生物过程中起作用的蛋白质或小多肽。MEROPS数据库中的I84蛋白酶抑制剂家族代表了一个新的蛋白酶抑制剂家族，已在2种双壳类，Crassostrea virginica和Sinonovacula constricta中报道，并被认为在宿主防御中发挥作用。本研究通过cDNA克隆技术，在mRNA水平上鉴定了文蛤（Meretrix meretrix）、贻贝（Mytilus galloprovincialis）和皱纹盘鲍（Haliotis discus hannai）等2种双壳类和1种腹足类I84家族的7个新成员。新发现的基因的表达模式不同，在响应盐度胁迫和病原体相关的分子模式刺激，表明它们参与宿主防御相关物种。此外，对公共数据库中的序列数据的分析未揭示非软体动物中的任何家族I84蛋白酶抑制剂分子。结果表明，家族I84蛋白酶抑制剂可能是软体动物特异性的，构成了软体动物物种独特的宿主防御机制。
Protease inhibitors are proteins or small polypeptides functioning in numerous biological processes in all organisms. The I84 family of protease inhibitors in the MEROPS database represents a novel protease inhibitor family that has been reported in 2 bivalves, Crassostrea virginica and Sinonovacula constricta, and is believed to play a role in host defense. In the present study, 7 new members of Family I84 were identified in 2 bivalves, Meretrix meretrix and Mytilus galloprovincialis, and 1 gastropod, Haliotis discus hannai, at the mRNA level via cDNA cloning. The expression patterns of the newly identified genes varied in response to salinity stresses and pathogen-associated molecular pattern stimulations, suggesting their involvement in the host defense of related species. Additionally, analyses of sequence data in public databases did not reveal any Family I84 protease inhibitor molecules in non-molluscan animals. The results indicated that Family I84 protease inhibitors are likely mollusk specific, constituting a unique host defense mechanism in molluscan species.