Beyond the cytoplasm of Escherichia coli: localizing recombinant proteins where you want them.
Beyond the cytoplasm of Escherichia coli: localizing recombinant proteins where you want them.
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超越大肠杆菌的细胞质：将重组蛋白定位在您想要的位置。
DOI：
10.1007/978-1-4939-2205-5_5
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
DeLisa,MatthewP
中科院分区：
文献类型：
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作者：
Boock,JasonT;Waraho-Zhmayev,Dujduan;Mizrachi,Dario;DeLisa,MatthewP
重组蛋白在大肠杆菌中的表达是生物技术事业的基石。虽然在这种宿主中的细胞质表达受到了最多的关注，但在这种隔室中获得大量正确折叠的蛋白质有时会遇到困难。这些问题通常可以通过将蛋白质表达靶向细胞质外区室（例如，膜、周质）或至培养基。本章讨论了将蛋白质从细胞质中输出的各种策略，以及用于监测和优化这些不同输出机制的工具。
Recombinant protein expression inEscherichia colirepresents a cornerstone of the biotechnology enterprise. While cytoplasmic expression in this host has received the most attention, achieving substantial yields of correctly folded proteins in this compartment can sometimes be met with difficulties. These issues can often be overcome by targeting protein expression to extracytoplasmic compartments (e.g., membrane, periplasm) or to the culture medium. This chapter discusses various strategies for exporting proteins out of the cytoplasm as well as tools for monitoring and optimizing these different export mechanisms.