Association of ADAM33 gene polymorphisms with asthma in Indian children
Association of ADAM33 gene polymorphisms with asthma in Indian children
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DOI：
10.1038/jhg.2010.157
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发表时间：
2011-03-01
影响因子：
3.5
通讯作者：
Husain, Nuzhat
中科院分区：
文献类型：
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作者：
Awasthi, Shally;Tripathi, Priya;Husain, Nuzhat
哮喘是儿童最常见的慢性疾病，哮喘加重是儿童发病和住院的重要原因。在本研究中，通过病例对照研究，研究了ADAM33基因单核苷酸多态(SNPs)与印度儿童哮喘的关系。采用聚合酶链式反应-限制性片段长度多态性方法，对211例1~15岁哮喘患者和137例正常对照的ADAM33基因F+1(Rs511898)G/A、S2(Rs528557)G/C、ST+4(Rs44707)A/C、ST+5(Rs597980)C/T和V4(Rs2787094)C/G 5个SNP进行了分析。数据采用x(2)检验和Logistic回归模型进行统计分析。使用期望最大化算法进行单倍型估计和连锁不平衡。ADAM33基因S2和ST+5单核苷酸多态与哮喘风险显著相关(P=0.020)。
Asthma is the most common chronic disorder in childhood, and asthma exacerbation is an important cause of childhood morbidity and hospitalization. In the present study, the relationship between single-nucleotide polymorphisms (SNPs) of the ADAM33 gene and asthma in Indian children has been examined using a case-control study. Five SNPs of the ADAM33 gene, F+1(rs511898) G/A, S2 (rs528557) G/C, ST+4 (rs44707) A/C, ST+5 (rs597980) C/T and V4 (rs2787094) C/G, were analyzed in 211 asthma cases and 137 controls aged 1-15 years using the PCR-restriction fragment length polymorphism method. Data were statistically analyzed using the x(2)-test and logistic regression model. Haplotype estimation and linkage disequilibrium were conducted using the expectation-maximization algorithm. The genotypes and allele frequencies of SNPs S2 and ST+5 of the ADAM33 gene were significantly associated with asthma risk (P=0.020-