Novel QTL for lodging resistance, PRL4, improves physical properties with high non-structural carbohydrate accumulation of basal culms in rice (Oryza sativa L.)
Novel QTL for lodging resistance, PRL4, improves physical properties with high non-structural carbohydrate accumulation of basal culms in rice (Oryza sativa L.)
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DOI：
10.1007/s10681-022-03036-6
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发表时间：
2022-06-01
期刊：
影响因子：
1.9
通讯作者：
Kashiwagi, Takayuki
中科院分区：
文献类型：
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作者：
Kashiwagi, Takayuki
水稻抗倒伏是水稻生产中的一个重要问题，水稻抗倒伏主要有三种类型：株高低、茎秆粗壮和植株下部强壮。与株高和秆力相比，下部强度的决定因素尚不清楚。本研究确定了一个新的遗传因素参与的强度较低的一部分，评估推阻力，使用染色体片段代换系（CSSL）澄清的CSSL和渗入线（IL）的功能分析的强度较低的一部分的决定因素，窝藏确定的数量性状位点（QTL）。QTL分析发现，增加推抗性的QTL在第4染色体上，PRL4，这是不相关的抽穗期。添加PRL 4的CSSL提高了基秆的抗推性和物理强度，但降低了实粒率和粒重。通过该CSSL回交开发的IL与PRL 4，提高了抗推性和压缩下的秆应变直到断裂，并且没有降低产量性状。这些品系与PRL 4增加积累的非结构性碳水化合物（NSC）在基秆在完全成熟阶段。因此，PRL 4对NSC在秆中积累的遗传控制可能通过增强秆的韧性和强度以及延展性来提高下部的强度。
Resistance to lodging, an important problem in rice production, has three types: low plant height, strong culm, and high strength of the lower part of the plant. The determinants of strength of the lower part remains unclear, compared with plant height and culm strength. This study identified a new genetic factor involved in the strength of the lower part, as assessed by pushing resistance, using chromosomal segment substitution lines (CSSLs) to clarify the determinants of strength of the lower part by functional analysis of the CSSL and the introgression line (IL) harboring the identified quantitative trait locus (QTL). QTL analysis identified the QTL for increasing pushing resistance on chromosome 4, PRL4, which was not related to days to heading. The CSSL with PRL4 showed increased pushing resistance and physical strength of the basal culm, but decreased filled grain ratio and grain weight. The IL with PRL4, developed by backcrossing this CSSL, improved pushing resistance and the strain of culm until breaking under compression, and did not decrease yield traits. These lines with PRL4 increased the accumulation of non-structural carbohydrate (NSC) in the basal culm at the fully ripe stage. Thus, the genetic control of NSC accumulation in culms by PRL4 may improve the strength of the lower part by enhancing culm toughness with strength and ductility.