SYNTHESIS OF RNA BY THE RAPID PHOSPHOTRIESTER METHOD USING AZIDO-BASED 2′-O-PROTECTING GROUPS
SYNTHESIS OF RNA BY THE RAPID PHOSPHOTRIESTER METHOD USING AZIDO-BASED 2′-O-PROTECTING GROUPS
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DOI：
10.1080/15257770903170286
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发表时间：
2009-01-01
影响因子：
1.3
通讯作者：
Chakhmakhcheva, O. G.
中科院分区：
文献类型：
--
作者：
Efimov, V. A.;Aralov, A. V.;Chakhmakhcheva, O. G.
报道了以叠氮甲基和2-（叠氮甲基）苯甲酰基为2 ′-OH保护基，O-亲核分子内催化磷酸三酯固相法合成寡核糖核苷酸。发展了合成相应的单体二叠氮基的方法，并考察了2 ′-O-叠氮甲基和2 ′-O-2（叠氮甲基）苯甲酰基在合成链长为15-22个核苷酸的不同RNA片段中的应用。叠氮甲基被发现是更优选的有效合成的寡核糖核苷酸。在去除2 '-O-叠氮甲基之前和之后，检查RNA对其互补寡核苷酸的杂交特性。
The azidomethyl and 2-(azidomethyl) benzoyl as 2'-OH protecting groups are reported for preparation of oligoribonucleotides by the phosphotriester solid-phase method using O-nucleophilic intramolecular catalysis. The procedures for the synthesis of the corresponding monomer synthons were developed and the usefulness of the application of 2'-O-azidomethyl and 2'-O-2(azidomethyl) benzoyl groups was examined in the synthesis of different RNA fragments with a chain length of 15-22 nucleotides. The azidomethyl group was found to be more preferable for effective synthesis of oligoribonucleotides. Hybridization properties of RNAs toward their complementary oligonucleotides were examined before and after the removal of 2'-O-azidomethyl groups.