Low carbohydrate ketogenic diet prevents the induction of diabetes using streptozotocin in rats
Low carbohydrate ketogenic diet prevents the induction of diabetes using streptozotocin in rats
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DOI：
10.1016/j.etp.2010.05.008
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发表时间：
2011-11-01
影响因子：
--
通讯作者：
Dashti, H.
中科院分区：
文献类型：
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作者：
Al-Khalifa, A.;Mathew, T. C.;Dashti, H.
糖尿病仍然是一种极其普遍的内分泌紊乱，导致一些微观和宏观并发症。人们普遍认为，饮食习惯的改变可以诱导或预防糖尿病的发生。研究表明，低碳水化合物生酮饮食（LCKD）在改善糖尿病的许多有害后果方面是有效的。然而，它在预防糖尿病发病中的作用尚不清楚。因此，本研究通过生化和组织学方法，重点研究LCKD在预防链脲佐菌素（STZ）诱导的大鼠糖尿病中的作用。本研究中使用了42只体重为150-250 g的Wistar大鼠。动物分为三组：正常饮食（ND）、低碳水化合物生酮饮食（LCKD）和高碳水化合物饮食（HCD）。向每组动物提供特定饲料，供其自由采食，持续8周。每组又分为正常对照组、假手术对照组和糖尿病组。糖尿病组动物单次腹腔注射STZ（55 mg/kg）。注射STZ 4周后处死动物。在整个12周的实验期间，每天测量食物和水的摄入量，并每周测量体重。注射STZ后，除LCKD组外，其余各组的血糖水平均显著升高（p < 0.01）。此外，除LCKD组外，所有组的摄食量、饮水量和尿量均显著增加（p值< 0.01）。与其他组相比，喂食LCKD的动物的体重增加也显著降低（p值< 0.05）。虽然在糖尿病大鼠中观察到β细胞数量的显著减少，但与LCKD对照组相比，LCKD治疗的糖尿病动物中的β细胞数量没有变化。因此，本研究的结果表明，LCKD可以预防大鼠使用链脲佐菌素发生糖尿病。(C)2010年Elsevier GmbH。All rights reserved.
Diabetes continues to be an overwhelmingly prevalent endocrine disorder that leads to several micro- and macrocomplications. It has been widely accepted that changes in dietary habits could induce or prevent the onset of diabetes. It is shown that low carbohydrate ketogenic diet (LCKD) is effective in the amelioration of many of the deleterious consequences of diabetes. However, its role in preventing the onset of diabetes is not understood. Therefore, this study is focused on the effect of LCKD in preventing the induction of diabetes using streptozotocin (STZ) in rats by biochemical and histological methods. Forty-two Wistar rats weighing 150-250 g were used in this study. The animals were divided into three groups: normal diet (ND), low carbohydrate ketogenic diet (LCKD), and high carbohydrate diet (HCD). Specific diets ad libitum were given to each group of animals for a period of 8 weeks. Each group was further subdivided into normal control, sham control and diabetic groups. Animals in the diabetic group were given a single intraperitoneal injection of STZ (55 mg/kg). All the animals were sacrificed 4 weeks after the injection of STZ. Daily measurements of food and water intake as well as weekly measurement of body weight were taken during the whole 12 weeks of the experiment. After injecting with STZ, the blood glucose level of all the groups increased significantly except for the group fed on LCKD (p value < 0.01). Also, food intake, water intake and urine output were significantly increased in all groups except for the LCKD group (p value < 0.01). There was also a significant decrease in the weight gain of the animals that were fed on a LCKD as compared to other groups (p value < 0.05). Although, substantial decrease in the number of beta cells was noticed in diabetic rats, there were no change in the number of beta cells in the LCKD treated diabetic animals as compared to LCKD control group. The results presented in this study, therefore, suggests that LCKD prevents the development of diabetes using streptozotocin in rats. (C) 2010 Elsevier GmbH. All rights reserved.