The degree of polymerization of inulin-like fructans affects cecal mucin and immunoglobulin A in rats.
The degree of polymerization of inulin-like fructans affects cecal mucin and immunoglobulin A in rats.
复制标题
菊粉样果聚糖的聚合度影响大鼠的盲肠粘蛋白和免疫球蛋白A。
作者：
H. Ito;T. Wada;M. Ohguchi;K. Sugiyama;S. Kiriyama;T. Morita
通过饲喂聚合度（DP）不同的菊糖型果聚糖的Wistar（WS）或Sprague-Dawley（SD）大鼠的盲肠发酵模式，检查盲肠粘蛋白和免疫球蛋白A（伊加）的量。以60 g/kg饲料饲喂动物10 d，饲喂对照饲料或含有平均DP为4、8、16或23的果聚糖之一的饲料。盲肠发酵产物之间的WS和SD大鼠喂养DP 8果聚糖，短链脂肪酸（SCFAs）作为主要的有机酸在前者，但乳酸占主导地位，在后者有很大的不同。两种菌株中果聚糖的盲肠发酵能力一般随着果聚糖DP的增加而降低，这尤其表现在DP 16和23中乳酸的量减少。在WS大鼠中，盲肠粘蛋白和伊加在所有果聚糖组均大于对照组。在SD大鼠中，与对照组相比，盲肠粘蛋白仅在DP 8、16和23组中更大，而伊加在DP 4和8组中更大。在这两种菌株中，盲肠粘蛋白与盲肠SCFA的总和相关，但与乳酸、琥珀酸或总有机酸无关。相反，只有盲肠乳酸与盲肠伊加在两个菌株。本研究表明，不同的发酵模式的果聚糖影响盲肠粘蛋白和伊加;粘蛋白很可能是响应盲肠SCFA的生产，而伊加增加时，发酵发生迅速，乳酸是一个主要的发酵产物。
Cecal amounts of mucin and immunoglobulin A (IgA) were examined through the cecal fermentation pattern in Wistar (WS) or Sprague-Dawley (SD) rats fed inulin-type fructans differing in their degree of polymerization (DP). The animals were fed a control diet or a diet containing one of the fructans with an average DP of 4, 8, 16, or 23, at 60 g/kg diet for 10 d. Cecal fermentation products substantially differed between WS and SD rats fed DP8 fructan, with short-chain fatty acids (SCFAs) as the major organic acids in the former but lactate predominating in the latter. Cecal fermentability of fructans in both strains generally decreased with increasing DP of fructans, and this was especially manifest in reduction of the amounts of lactate in DP16 and 23. In WS rats, cecal mucin and IgA were greater in all fructan groups than in the control group. In SD rats, cecal mucin was greater only in the DP8, 16, and 23 groups as compared to the control group, while IgA was greater in the DP4 and 8 groups. In both strains, cecal mucin correlated with the sum of cecal SCFAs, but not with lactate, succinate, or total organic acids. In contrast, only cecal lactate correlated with cecal IgA in both strains. The present study shows that the different fermentation patterns of fructans affect cecal mucin and IgA; mucin is likely to respond to cecal SCFA production, whereas IgA increases when fermentation occurs rapidly and lactate is a major fermentation product.