Regulation of the relationship between phytoplankton Scenedesmus acutus and heterotrophic bacteria by the balance of light and nutrients
Regulation of the relationship between phytoplankton Scenedesmus acutus and heterotrophic bacteria by the balance of light and nutrients
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DOI：
10.3354/ame017027
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发表时间：
1999-04-30
影响因子：
1.4
通讯作者：
Nakanishi, M
中科院分区：
文献类型：
--
作者：
Gurung, TB;Urabe, J;Nakanishi, M
通过培养实验，研究了在光照和营养平衡条件下，尖生栅藻和异养细菌之间的关系是否发生了变化。在高氮磷比（80：1）条件下，藻菌混合物在6种光照强度和4种磷浓度的不同组合下生长。我们使用由无机营养物组成的人工培养基，使细菌依赖于藻类释放的有机物作为碳（C）源。每2天，用新鲜培养基替换25%的培养物悬浮液。在培养结束时，当细菌和藻类密度都稳定时，将细菌与藻类分离。然后进行葡萄糖和/或无机P富集的生物测定，以评估有机C或无机P对细菌生长速率的限制程度。半分批培养中的藻类密度在光照强度下较低
Culture experiments were conducted with the alga Scenedesmus acutus and heterotrophic bacteria to examine if the nature of their relationship changes according to the balance of light and nutrient supplies. Mixtures of algae and bacteria were grown in various combinations of 6 light intensities and 4 phosphorus (P) concentrations at high N:P ratio (80:1). We used an artificial medium composed of inorganic nutrients so that bacteria relied on organic matter released by algae as carbon (C) source. Every 2 d, 25 % of the culture suspension was replaced by fresh medium. At the end of incubation when both bacterial and algal densities were stabilized, bacteria were separated from algae. Bioassays with glucose and/or inorganic P enrichment were then performed to assess the extent to which bacterial growth rate was limited by organic C or inorganic P. The algal density in the semibatch culture was low under the light intensity