3D analysis of enamel demineralisation in human dental caries using high-resolution, large field of view synchrotron X-ray micro-computed tomography
3D analysis of enamel demineralisation in human dental caries using high-resolution, large field of view synchrotron X-ray micro-computed tomography
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DOI：
10.1016/j.mtcomm.2021.102418
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发表时间：
2021-05-19
影响因子：
3.8
通讯作者：
Korsunsky, Alexander M.
中科院分区：
文献类型：
--
作者：
Besnard, Cyril;Harper, Robert A.;Korsunsky, Alexander M.
我们报告的主要进展同步加速器3D数据集从人类健康和龋牙釉质的分析。收集了三个人类龋齿样本和一个非龋齿参考牙齿样本的同步加速器断层扫描数据，体素尺寸为325 nm，总体积为815.4 x 815.4 x 685.4 μ m（3）。结果进行了比较，与传统的X射线断层扫描，光学显微镜，聚焦离子束扫描电子显微镜。与常规断层扫描相比，同步辐射断层扫描由于矿物质含量减少而在脱矿釉质中观察到明显的对比。发现这些特征与棱柱状釉质内的杆和杆间结构相对应。2D和3D图像分割允许重要结构特征的统计量化（例如空隙的纵横比和横截面积，以及作为病变深度的函数的脱矿体积分数）。虽然整体龋坏牙釉质主要显示1型蚀刻模式（优先脱矿的釉质棒），2型（优先杆间脱矿）和1型之间的过渡被确定在同一病变的第一次。这项研究并没有提供广泛的结果，对不同的病变研究，但说明了一种新的方法及其潜在的应用。
We report major advances in the analysis of synchrotron 3D datasets acquired from human healthy and carious dental enamel. Synchrotron tomographic data for three human carious samples and a non-carious reference tooth sample were collected with the voxel size of 325 nm for a total volume of 815.4 x 815.4 x 685.4 mu m(3). The results were compared with conventional X-ray tomography, optical microscopy, and focused ion beam-scanning electron microscopy. Clear contrast was seen within demineralised enamel due to reduced mineral content using synchrotron tomography in comparison with conventional tomography. The features were found to correspond with the rod and inter-rod structures within prismatic enamel. 2D and 3D image segmentation allowed statistical quantification of important structural characteristics (such as the aspect ratio and the cross-sectional area of voids, as well as the demineralised volume fraction as a function of lesion depth). Whilst overall carious enamel predominantly displayed a Type 1 etching pattern (preferential demineralisation of enamel rods), a transition between Type 2 (preferential inter-rod demineralisation) and Type 1 was identified within the same lesion for the first time. This study does not provide extensive results on the different lesions studied, but illustrate a new method and its potential application.