Quantitative assessment of brain glucose metabolic rates using in vivo deuterium magnetic resonance spectroscopy
Quantitative assessment of brain glucose metabolic rates using in vivo deuterium magnetic resonance spectroscopy
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DOI：
10.1177/0271678x17706444
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发表时间：
2017-11-01
影响因子：
6.3
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Lu, Ming;Zhu, Xiao-Hong;Chen, Wei
脑葡萄糖消耗率（CMRglc）和三羧酸循环流量（V-TCA）的定量评估是了解生理病理条件下的神经能量学的关键。在这项研究中，我们报告了一种新的在体内氘（H-2）MRS（DMRS）的方法，同时测量和定量CMRglc和V-TCA在大鼠大脑在16.4特斯拉。短暂输注氘代葡萄糖后，同位素标记的葡萄糖，谷氨酸/谷氨酰胺（Glx）和水含量在大脑中的动态变化可以从其良好分辨的H-2共振鲁棒地监测。采用动态DMRS葡萄糖和Glx数据同时测定CMRglc和V-TCA。为了测试这种方法对葡萄糖代谢改变的敏感性，研究了两种不同麻醉剂的大脑状况。与使用2%异氟烷进行深度麻醉相比，在吗啡下大鼠中发现CMRglc（0.46 vs. 0.28 mmol/g/min）和V-TCA（0.96 vs. 0.6 mmol/g/min）增加。本研究证明了体内DMRS方法在高/低场强下评估脑葡萄糖代谢率的可行性和新实用性。它为在体研究生理病理状态下脑内葡萄糖有氧代谢和无氧代谢之间的代谢耦合关系提供了一种替代的MRS工具。
Quantitative assessment of cerebral glucose consumption rate (CMRglc) and tricarboxylic acid cycle flux (V-TCA) is crucial for understanding neuroenergetics under physiopathological conditions. In this study, we report a novel in vivo Deuterium (H-2) MRS (DMRS) approach for simultaneously measuring and quantifying CMRglc and V-TCA in rat brains at 16.4 Tesla. Following a brief infusion of deuterated glucose, dynamic changes of isotope-labeled glucose, glutamate/glutamine (Glx) and water contents in the brain can be robustly monitored from their well-resolved H-2 resonances. Dynamic DMRS glucose and Glx data were employed to determine CMRglc and V-TCA concurrently. To test the sensitivity of this method in response to altered glucose metabolism, two brain conditions with different anesthetics were investigated. Increased CMRglc (0.46 vs. 0.28 mmol/g/min) and V-TCA (0.96 vs. 0.6 mmol/g/min) were found in rats under morphine as compared to deeper anesthesia using 2% isoflurane. This study demonstrates the feasibility and new utility of the in vivo DMRS approach to assess cerebral glucose metabolic rates at high/ultrahigh field. It provides an alternative MRS tool for in vivo study of metabolic coupling relationship between aerobic and anaerobic glucose metabolisms in brain under physiopathological states.