Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipidsin symbiotic Lactobacillus, Staphylococcus and Streptococcus species.
Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipidsin symbiotic Lactobacillus, Staphylococcus and Streptococcus species.
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细菌种类-分子种类的特征谱，以及共生乳杆菌、葡萄球菌和链球菌种类的磷脂和糖脂的抗原性。
DOI：
10.1007/s10719-012-9393-2
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发表时间：
2012
期刊：
影响因子：
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et al.
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文献类型：
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作者：
Masao Iwamori;et al.
人类的共生细菌，肠道中的罗伊氏乳杆菌（LR），皮肤中的表皮葡萄球菌（SE）和口腔中的唾液链球菌（SS），都含有二己基二甘油酯（DH-DG），其浓度相当于磷脂酰甘油（PG）和心磷脂（CL），以及单至四己基二甘油酯。作为DG部分脂肪酸组合的分子种类显示出细菌的种类特征，但单个细菌的糖脂和磷脂之间具有相似性，LR为16:0和cy19:0， SE为ai15:0和ai17:0， SS为16:0和18:1。dh - dg的碳水化合物结构也具有细菌物种特征，LR的碳水化合物结构为Galα1-2Glcα， SE的碳水化合物结构为Glcβ1-6Glcβ， SS的碳水化合物结构为Glcα1-2Glcα。此外，研究还发现，细菌糖脂在兔免疫过程中提供了不同细菌种类的抗原决定因子。Anti-L。johnson抗血清与三烷基和四己基dg发生强烈反应，其中Galα通过α1-6键与DH-DG结合，并与LR中的DH-DG结合。虽然抗SE抗血清优先与SE的DH-DG反应，但抗SS抗血清与SS的DH-DG反应，与LR和SE的DH-DG反应程度较低。但是，抗se和抗ss抗血清与非还原末端具有相同碳水化合物的单己基DG或鞘糖脂完全不发生反应。此外，75%的人血清，无论ABO血型，被发现含有IgM来自LR的三和四己基DGs，但不包括来自LR， SE和SS的DH-DGs。
Human symbiotic bacteria,Lactobacillus reuteri(LR) in the intestines,Staphylococcus epidermidis(SE) in skin andStreptococcus salivalis(SS) in the oral cavity, contain dihexaosyl diglycerides (DH-DG) in concentrations equivalent to those of phosphatidyl glycerol (PG) and cardiolipin (CL), together with mono- to tetrahexaosyl DGs. The molecular species, as the combination of fatty acids in the DG moiety, were revealed to be bacterial species-characteristic, but to be similar between glycolipids and phospholipids in individual bacteria, the major ones being 16:0 and cy19:0 for LR, ai15:0 and ai17:0 for SE, and 16:0 and 18:1 for SS, respectively. The carbohydrate structures of DH-DGs were also bacterial species-characteristic, being Galα1-2Glcα for LR, Glcβ1-6Glcβ for SE, and Glcα1-2Glcα for SS, respectively. Also, bacterial glycolipids were revealed to provide antigenic determinants characteristic of bacterial species on immunization of rabbits with the respective bacteria. Anti-L. johnsoniiantiserum intensely reacted with tri- and tetrahexaosyl DGs, in which Galα was bound to DH-DG through an α1-6 linkage, as well as with DH-DG from LR. Although anti-SE antiserum preferentially reacted with DH-DG from SE, anti-SS antiserum reacted with DH-DG from SS and, to a lesser extent, with DH-DGs from LR and SE. But, both anti-SE and anti-SS antiserum did not react at all with monohexaosyl DG or glycosphingolipids with the same carbohydrates at the nonreducing terminals. In addition, 75 % of human sera, irrespective of the ABO blood group, were found to contain IgM to tri- and tetrahexaosyl DGs from LR, but not to DH-DGs from LR, SE and SS.