Positively Charged Tags Impede Protein Mobility in Cells as Quantified by 19F NMR.
Positively Charged Tags Impede Protein Mobility in Cells as Quantified by 19F NMR.
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DOI：
10.1021/acs.jpcb.9b02162
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发表时间：
2019-05
期刊：
影响因子：
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通讯作者：
Yansheng Ye;Qiong Wu;Wenwen Zheng;Bin Jiang;G. Pielak;Maili Liu;Conggang Li
中科院分区：
文献类型：
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作者：
Yansheng Ye;Qiong Wu;Wenwen Zheng;Bin Jiang;G. Pielak;Maili Liu;Conggang Li
蛋白质通常被标记用于可视化或在细胞中的其他大分子的“海洋”中递送，但标签如何影响蛋白质流动性仍然知之甚少。在这里，我们采用细胞内19 F NMR量化的蛋白质的流动性与带电的标签在大肠杆菌细胞和非洲爪蟾卵母细胞。我们发现，标签和细胞成分之间的瞬时电荷-电荷相互作用影响蛋白质的流动性。更具体地说，带正电荷的标签阻碍蛋白质的流动性。
Proteins are often tagged for visualization or delivery in the "sea" of other macromolecules in cells but how tags affect protein mobility remains poorly understood. Here, we employ in-cell 19F NMR to quantify the mobility of proteins with charged tags in Escherichia coli cells and Xenopus laevis oocytes. We find that the transient charge-charge interactions between the tag and cellular components affect protein mobility. More specifically, positively charged tags impede protein mobility.