Confocal Scanning Laser Fluorescence And Reflection Microscopy: Measurements Of The 3-D Image Formation And Applications In Biology

Confocal Scanning Laser Fluorescence And Reflection Microscopy: Measurements Of The 3-D Image Formation And Applications In Biology
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共焦扫描激光荧光和反射显微镜:3D 图像形成的测量及其在生物学中的应用

DOI:
10.1117/12.941510
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发表时间:
1987
期刊:
Other Conferences
影响因子:
--
通讯作者:
N. Nanninga
N. Nanninga
中科院分区:
--
文献类型:
--
作者:
H. V. D. van der Voort;G. Brakenhoff;C. Janssen;J. Valkenburg;N. Nanninga

文献摘要

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描述了一种直接耦合到图像处理系统的同轴型共焦扫描激光显微镜。的实际响应函数的测量结果,无论是在荧光和反射。结合图像处理的共聚焦技术在生物学领域的应用进行了演示。
A confocal scanning laser microscope of the on-axis type, directly coupled to an image processing system is described. Results of measurements of the actual response functions, both in fluorescence and reflection are presented. Applications of the confocal technique in the area of biology in combination with image processing are demonstrated.