Transparent magnetic photoresists for bioanalytical applications.
Transparent magnetic photoresists for bioanalytical applications.
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DOI：
10.1016/j.biomaterials.2010.07.087
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发表时间：
2010-11
期刊：
影响因子：
14
通讯作者：
Allbritton, Nancy L.
中科院分区：
文献类型：
--
作者：
Gach, Philip C.;Sims, Christopher E.;Allbritton, Nancy L.
具有磁性的微细加工器件由于可以利用外部磁铁进行控制，在生物分析微器件中具有很大的应用价值。目前制造磁性微器件的方法产生一种低透明度材料，防止基于光学显微镜对结构上的生物样品进行检查。均匀透明的磁性光致抗蚀剂是为需要高透明度以及整个结构的一致磁性的微型器件开发的。在添加负性光致抗蚀剂SU-8和1002F的过程中，通过对纳米颗粒进行有机覆盖和使用溶剂型分散技术，可以最大限度地减少10 nm磁铁矿颗粒的胶体形成。透射电子显微镜观察到，当磁铁矿浓度为0.01%~1%时，由于磁铁矿纳米粒子在聚合物中均匀分散，光致抗蚀剂具有较高的透明度。这些磁性光刻胶被用来制作长宽比高达4：1、分辨率为3μm的微结构。各种细胞系在磁性光刻胶上表现出良好的粘附性和生存能力。用透射电子显微镜对培养在磁性光刻胶上的细胞进行检查，发现细胞摄取了从光刻胶中提取的磁性纳米颗粒。通过用天然1002F光刻胶覆盖磁性光刻胶表面或通过表面粗糙处理去除磁性光刻胶的顶层，可以消除磁性纳米颗粒对细胞的污染。通过对培养在可释放磁性微盘阵列上的单个细胞(HeLa、RBL和3T3细胞)的分选，证明了这些磁性光刻胶的实用性。具有附着细胞的磁性微托盘在从阵列释放后被收集100%。85-92%的收集细胞扩增成集落。聚合物磁性材料应该在生物分析技术的微结构制造中得到广泛的应用。
Microfabricated devices possessing magnetic properties are of great utility in bioanalytical microdevices due to their controlled manipulation with external magnets. Current methods for creating magnetic microdevices yield a low-transparency material preventing light microscopy-based inspection of biological specimens on the structures. Uniformly transparent magnetic photoresists were developed for microdevices that require high transparency as well as consistent magnetism across the structure. Colloidal formation of 10 nm maghemite particles was minimized during addition to the negative photoresists SU-8 and 1002F through organic capping of the nanoparticles and utilization of solvent-based dispersion techniques. Photoresists with maghemite concentrations of 0.01 to 1% had a high transparency due to the even dispersal of maghemite nanoparticles within the polymer as observed with transmission electron microscopy (TEM). These magnetic photoresists were used to fabricate microstructures with aspect ratios up to 4:1 and a resolution of 3 μm. Various cell lines showed excellent adhesion and viability on the magnetic photoresists. An inspection of cells cultured on the magnetic photoresists with TEM showed cellular uptake of magnetic nanoparticles leeched from the photoresists. Cellular contamination by magnetic nanoparticles was eliminated by capping the magnetic photoresist surface with native 1002F photoresist or by removing the top layer of the magnetic photoresist through surface roughening. The utility of these magnetic photoresists was demonstrated by sorting single cells (HeLa, RBL and 3T3 cells) cultured on arrays of releasable magnetic micropallets. 100% of magnetic micropallets with attached cells were collected following release from the array. 85–92% of the collected cells expanded into colonies. The polymeric magnetic materials should find wide use in the fabrication of microstructures for bioanalytical technologies.
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