Tissue culture: the unlimited potential
Tissue culture: the unlimited potential
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DOI：
10.1007/s11626-010-9315-1
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发表时间：
2010-05
期刊：
影响因子：
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通讯作者：
Gordon H. Sato;J. Sato;T. Okamoto;W. McKeehan;David W. Barnes
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文献类型：
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作者：
Gordon H. Sato;J. Sato;T. Okamoto;W. McKeehan;David W. Barnes
在组织培养中缺乏细胞的组织特异性分化功能，曾经被认为是由于“去分化”，通过一系列挑战流行教条的简单实验表明是由于成纤维细胞的选择性过度生长。根据这一认识，设计了富集培养技术（交替动物和培养传代），以使功能分化的肿瘤细胞相对于成纤维细胞具有选择性优势。这些实验导致了大量功能分化的克隆株的衍生范围内的细胞类型，提供了最终点的破坏教条的“去分化”。相反，提出的假设是，培养中的细胞准确地代表体内细胞，但没有复杂的体内环境。随着植物成分确定培养基的发展及其与功能分化克隆细胞系的结合，这一概念得到了加强，组织培养研究的潜力也得到了极大的增强。激素确定的培养基允许不能在常规的血清补充培养基中生长的细胞类型的培养。这些方法表明，激素反应和依赖性可以在文化中发现。遵循这种想法和癌细胞激素依赖性的发现为治疗带来了新的理论基础。组织培养和细胞技术在解决人类健康问题方面继续发挥重要作用。
Lack of tissue-specific differentiated functions of cells in tissue culture, once thought to be due to “dedifferentiation”, was shown to be due to selective overgrowth of fibroblasts by a series of simple experiments that challenged the prevailing dogma. Following this insight, enrichment culture techniques (alternate animal and culture passage) were designed to give functionally differentiated tumor cells selective advantage over the fibroblasts. These experiments resulted in the derivation of a large number of functionally differentiated clonal strains of a range of cell types, providing the final point of destruction of the dogma of “dedifferentiation.” Instead, the hypothesis was proposed that cells in culture accurately represent cells in vivo, but without the complex in vivo environment. With the development of hormonally defined media and its combination with functionally differentiated clonal cell lines, this concept has been strengthened and the potential of tissue culture studies has been greatly augmented. Hormonally defined media allow the culture of cell types that cannot be grown in conventional, serum-supplemented media. These approaches demonstrate that hormonal responses and dependencies can be discovered in culture. Following this thinking and the discovery of hormonal dependencies of cancer cells has led to a new rationale for therapy. Tissue culture and cell technology continue to play an important role in solving human health problems.