BASAL MEMBRANE-ASSOCIATED SUBSTANCES IN HUMAN SALIVARY-GLANDS AND SALIVARY-GLAND TUMORS
BASAL MEMBRANE-ASSOCIATED SUBSTANCES IN HUMAN SALIVARY-GLANDS AND SALIVARY-GLAND TUMORS
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DOI：
10.1016/s0344-0338(88)80084-0
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发表时间：
1988-08-01
影响因子：
2.8
通讯作者：
SCHUPPAN, D
中科院分区：
文献类型：
--
作者：
CASELITZ, J;SCHMITT, P;SCHUPPAN, D
分析了人唾液腺组织中基底膜相关物质的分布。用单克隆抗体检测IV型胶原蛋白和层粘连蛋白，用多克隆抗体检测纤维连接蛋白。正常唾液腺组织基底膜结构保存完好。腺泡和导管周围的基膜连续染色。这些物质的标记似乎与肌上皮细胞有关。多形性腺瘤表现出基底膜物质的异质性。局部，IV型胶原蛋白和层粘连蛋白以及纤维连接蛋白增加。这在邻近的肌上皮样细胞中观察到。多形性腺瘤的其他部位基底膜中断。腺样囊性癌假性囊肿基底膜相关物质清晰染色。间质小梁呈不规则染色。腺泡细胞瘤、腺癌、黏液表皮样瘤和鳞状细胞癌（与头颈部其他区域相比）通过抗IV型胶原和层粘连蛋白抗体可见基底膜明显破坏。基础膜物质的研究可能有助于识别唾液腺肿瘤的特殊特征，并对恶性肿瘤的侵袭行为进行分级。
Human salivary gland tissue was analysed with respect to the distribution of basal membrane associated substances. Collagen type IV and laminin were studied on the basis of monoclonal antibodies, fibronectin was analysed with polyclonal antibodies. The structure of the basal membrane was well preserved in normal salivary gland tissue. There was a continuous staining of the basal membrane around the acini and the ducts. The labelling for these substances appeared to be associated with the myoepithelial cells. Pleomorphic adenomas exhibited a heterogeneous pattern for the basal membrane substances. Focally, there was an augmentation of collagen IV and laminin, as well as of fibronectin. This was observed in the neighbourhood of myoepithelial like cells. Other parts of pleomorphic adenomas showed an interruption of the basal membrane. Adenoid cystic carcinomas displayed a clear staining of the basal membrane associated substances in the pseudocysts. Stromal trabeculae were stained in an irregular manner. Acinic cell tumours, adenocarcinomas, mucoepidermoid tumours and squamous cell carcinomas (for comparison taken from other regions in the head and neck area) presented a clear destruction of the basal membrane as visualized by antibodies against collagen IV and laminin. The study of the basal mambrane substances may be helpful for identifying special features of salivary gland tumours and for grading the amount of invasive behaviour in the malignant tumours.