Mobility of the hepatitis C virus NS4B protein on the endoplasmic reticulum membrane and membrane-associated foci
Mobility of the hepatitis C virus NS4B protein on the endoplasmic reticulum membrane and membrane-associated foci
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DOI：
10.1099/vir.0.80768-0
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发表时间：
2005-05-01
影响因子：
3.8
通讯作者：
McLauchlan, J
中科院分区：
文献类型：
--
作者：
Gretton, SN;Taylor, AI;McLauchlan, J
丙型肝炎病毒 (HCV) 非结构蛋白 NS4B 会诱导内质网 (ER) 膜的形态变化，这可能在病毒 RNA 复制中发挥直接作用。嵌合 GFP-NS4B 融合蛋白位于 ER 膜和附着于 ER 的病灶处。这些膜相关病灶 (MAF) 可能与复制 HCV RNA 的细胞中观察到的膜变化有关。 MAF 的关系；与预先存在的细胞结构的关系尚不清楚。间接免疫荧光分析表明，它们不含有囊泡的细胞标记，而囊泡与其他病毒的复制有关。从光漂白研究到检查 NS4B 的扩散，与 ER 膜上相比，GFP 标记的蛋白在 MAF 上的迁移率降低。这种较慢的迁移率表明 NS4B 可能在 MAF 和 ER 上形成不同的相互作用。
The hepatitis C virus (HCV) non-structural protein NS4B induces morphological changes in the endoplasmic reticulum (ER) membrane that may have a direct role in viral RNA replication. A chimeric GFP-NS4B fusion protein located to the ER membrane and to foci that were attached to the ER. These membrane-associated foci (MAFs) could be related to the membrane alterations observed in cells that replicate HCV RNA. The relationship of MAFs; to pre-existing cellular structures is not known. Indirect immunofluorescence analysis demonstrated that they did not contain a cellular marker for vesicles, which have been implicated in the replication of other viruses. From photobleaching studies to examine diffusion of NS4B, the GFP-tagged protein had reduced mobility on MAFs compared with on the ER membrane. This slower mobility suggested that NS4B is likely to form different interactions on MAFs and the ER.