Development of a Basement Membrane Substitute Incorporated Into an Electrospun Scaffold for 3D Skin Tissue Engineering
Development of a Basement Membrane Substitute Incorporated Into an Electrospun Scaffold for 3D Skin Tissue Engineering
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DOI：
10.1166/jbt.2014.1224
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发表时间：
2014-09-01
影响因子：
0.1
通讯作者：
MacNeil, Sheila
中科院分区：
文献类型：
--
作者：
Bye, Frazer J.;Bullock, Anthony J.;MacNeil, Sheila
3D组织工程皮肤生产中的一个主要挑战是基底膜区域的重建，以促进角质形成细胞与浸渍有成纤维细胞的真皮替代物的牢固附着和分离。我们先前已经证明，简单的静电纺丝支架提供纤维，细胞在其上附着、增殖并自我分选成上皮和真皮。在本研究的一个发展中，三层支架然后由聚L-乳酸和聚羟基丁酸酯-共-羟基戊酸酯静电纺丝。在这些中，将包含纳米多孔/纳米纤维聚羟基丁酸酯-共-羟基戊酸酯纤维的支架的中心层交织到本体微孔聚L-乳酸微纤维中以模拟基底膜。接种到这些支架上并培养2周的角质形成细胞和成纤维细胞显示，两种细胞类型都不能穿过中心纳米多孔屏障（通过SEM显示，并用CellTracker（TM）进行荧光监测），而微纤维聚L-乳酸提供了一种支架，角质形成细胞可以在其上产生上皮，成纤维细胞可以产生沉积胶原的真皮替代物。虽然细胞没有穿透这个屏障，但细胞间的相互作用仍然是上皮发育所必需的。
A major challenge in the production of 3D tissue engineered skin is the recreation of the basement membrane region to promote secure attachment and yet segregation of keratinocytes from the dermal substitute impregnated with fibroblasts. We have previously shown that simple electrospun scaffolds provide fibres on which the cells attach, proliferate, and self-sort into epithelium and dermis. In a development of this in this study tri-layered scaffolds were then electrospun from poly L-lactic acid and poly hydroxybutyrate-co-hydroxyvalerate. In these a central layer of the scaffolds comprising nano-porous/nano-fibrous poly hydroxybutyrate-co-hydroxyvalerate fibres was interwoven into the bulk micro-porous poly L-lactic acid microfibers to mimic the basement membrane. Keratinocytes and fibroblasts seeded onto these scaffolds and cultured for 2 weeks showed that neither cell type was able to cross the central nano-porous barrier (shown by SEM, and fluorescence monitoring with CellTracker (TM)) while the micro-fibrous poly L-lactic acid provided a scaffold on which keratinocytes could create an epithelium and fibroblasts could create a dermal substitute depositing collagen. Although cells did not penetrate this barrier the interaction of cells was still evident-essential for epithelial development.