DEVELOPMENT OF AN RFLP LINKAGE MAP IN DIPLOID PEANUT SPECIES
DEVELOPMENT OF AN RFLP LINKAGE MAP IN DIPLOID PEANUT SPECIES
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DOI：
10.1007/bf01184927
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发表时间：
1993-11-01
影响因子：
5.4
通讯作者：
KOCHERT, G
中科院分区：
文献类型：
--
作者：
HALWARD, T;STALKER, HT;KOCHERT, G
花生的 RFLP 连锁图谱已开发用于遗传研究和育种计划，旨在改良栽培品种（Arachishypogaea L.）。源自花生组中两个相关二倍体物种（A. stenosperma x A. cardenasii）种间杂交的 F2 群体用于构建图谱。随机基因组克隆和 cDNA 克隆均用于开发图谱框架。此外，代表编码参与脂质生物合成途径的酶的基因的三个cDNA克隆已在花生中定位。在评估的 100 个基因组克隆和 300 个 cDNA 克隆中，分别有 15 个和 190 个揭示了我们作图群体的父母之间的多态性。不幸的是，其中大量产生了无法绘制的复杂带状图案。在分析分离的 132 个标记中，117 个分布在 11 个连锁群中，而 15 个尚未与任何其他标记关联。迄今为止，已覆盖的总地图距离约为 1063 厘米。
An RFLP linkage map of peanut has been developed for use in genetic studies and breeding programs aimed at improving the cultivated species (Arachis hypogaea L.). An F2 population derived from the interspecific hybridization of two related diploid species in the section Arachis (A. stenosperma x A. cardenasii) was used to construct the map. Both random genomic and cDNA clones were used to develop the framework of the map. In addition, three cDNA clones representing genes coding for enzymes involved in the lipid biosynthesis pathway have been mapped in peanut. Of the 100 genomic and 300 cDNA clones evaluated, 15 and 190, respectively, revealed polymorphisms among the parents of our mapping population. Unfortunately, a large number of these produced complex banding patterns that could not be mapped. Of the 132 markers analyzed for segregation, 117 are distributed among 11 linkage groups, while 15 have not yet been associated with any other marker. A total map distance of approximately 1063 cM has been covered to-date.