Reproducibility of Non-Invasive A1 Adenosine Receptor Quantification in the Rat Brain Using [18F]CPFPX and Positron Emission Tomography
Reproducibility of Non-Invasive A1 Adenosine Receptor Quantification in the Rat Brain Using [18F]CPFPX and Positron Emission Tomography
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DOI：
10.1007/s11307-014-0729-0
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发表时间：
2014-10-01
影响因子：
3.1
通讯作者：
Bauer, Andreas
中科院分区：
文献类型：
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作者：
Kroll, Tina;Elmenhorst, David;Bauer, Andreas
A（1）AR拮抗剂8-环戊基-3-（3-氟丙基）-1-丙基黄嘌呤（[F-18]CPFPX）最近被证明是一种适合于大鼠A（1）腺苷受体（A（1）AR）定量体内成像的放射性示踪剂。本研究评价了使用[F-18]CPFPX和专用小动物正电子发射断层扫描仪（PET）进行的非侵入性纵向A（1）AR研究的可重复性。A（1）AR可用性通过不同的非侵入性方法确定，包括基于简化和多线性参考组织（嗅球）的模型和图形方法。结果参数结合潜力（BP）的变异性和重现性进行了评价。重复估计的[F-18]CPFPX BP（ND）给出了可靠的结果，可接受的变异性（平均12%）和重现性（组内相关系数范围为0.57至0.68）在皮层和皮层下区域的大鼠脑。在动力学模型方面，简化参考组织模型（SRTM）的重测稳定性上级多线性和图形方法。使用[F-18]CPFPX PET对大鼠脑中A（1）AR密度进行非侵入性定量具有可重复性和可靠性，并允许对啮齿动物进行体内成像研究的纵向设计。
The A(1)AR antagonist 8-cyclopentyl-3-(3-fluoropropyl)-1-propylxanthine ([F-18]CPFPX) has recently been shown to be a suitable radiotracer for quantitative in vivo imaging of the A(1) adenosine receptor (A(1)AR) in rats. The present study evaluates the reproducibility of non-invasive longitudinal A(1)AR studies with [F-18]CPFPX and a dedicated small animal positron emission tomography (PET) scanner.Twelve male Sprague Dawley rats underwent four repeated dynamic PET scans with a bolus injection of [F-18]CPFPX. A(1)AR availability was determined by different non-invasive approaches including simplified and multilinear reference tissue (olfactory bulb)-based models and graphical methods. The outcome parameter binding potential (BP) was evaluated in terms of variability and reproducibility.Repeated estimations of [F-18]CPFPX BP (ND) gave reliable results with acceptable variability (mean 12 %) and reproducibility (intraclass correlation coefficients raging from 0.57 to 0.68) in cortical and subcortical regions of the rat brain. With regard to kinetic models, test-retest stability of the simplified reference-tissue model (SRTM) was superior to multilinear and graphical approaches.Non-invasive quantification of A(1)AR density in the rat brain is reproducible and reliable with [F-18]CPFPX PET and allows longitudinal designs of in vivo imaging studies in rodents.