Highly expansive DNA hydrogel films prepared with photocrosslinkable poly(vinyl alcohol)
Highly expansive DNA hydrogel films prepared with photocrosslinkable poly(vinyl alcohol)
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DOI：
10.1016/j.colsurfa.2005.11.027
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发表时间：
2006-08-15
影响因子：
5.2
通讯作者：
Ijiro, Kuniharu
中科院分区：
文献类型：
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作者：
Ishizuka, Noriko;Hashimoto, Yuichi;Ijiro, Kuniharu
脱氧核糖核酸（DNA）具有阴离子聚电解质、分子识别和缩合电子等诸多特性。因此，它不仅可以作为功能材料应用于生物领域，还可以作为功能材料应用于物理领域。在这篇报道中，我们试图通过混合DNA和光可连接聚合物来制备不溶于水的DNA膜，目的是制备生物功能化的水凝胶膜。为此，使用光交联聚乙烯醇（叠氮基悬垂水溶性光聚合物；AWP）。将DNA和AWP的水溶液浇铸在玻璃衬底上。紫外线照射若干分钟后，DNA和AWP之间通过光交联形成一层水凝胶膜。将形成的水凝胶膜在纯水中浸泡1天，观察到膜有较大的膨胀。将膨胀膜浸泡在NaCl或CTAB溶液中进行收缩。薄膜的膨胀和收缩机制归因于水在渗透压作用下渗透到薄膜与基体之间的空隙中。这种现象可以应用于凝胶装置的制造，例如，一种新的生物传感器和药物输送系统。(c) 2005 Elsevier B.V.版权所有
Deoxyribonucleic acid (DNA) has a lot of characteristic features, e.g., anionic polyelectrolyte, molecular recognition and condensed pi electron. Therefore, it is expected to be applied as functional materials in not only biological but also physical fields. In this report, we attempted to prepare water-insoluble DNA films by mixing DNA and photolinkable polymer, aiming at the fabrication of bio-functionalized hydrogel films. For this purpose, photocrosslinkable polyvinyl alcohol (azide-unit pendant water-soluble photopolymer; AWP) was used. An aqueous DNA and AWP mixture was cast onto glass substrates. Irradiation of UV light on it for certain minutes provided a hydrogel film by photocrosslinking between DNA and AWP. When the formed hydrogel film was soaked in pure water for I day, large expansion of the film was observed. The expanded film was shrunk by soaking it in aqueous NaCl or CTAB solutions. The expansion and shrinkage mechanism of the film was attributed to the penetration of water into the gap between the film and the substrate due to osmotic pressure. This phenomenon can be applied to the fabrication of gel devices, e.g., a novel biosensor and drug delivery system. (c) 2005 Elsevier B.V. All rights reserved.