Submillisecond AMPA receptor-mediated signaling at a principal neuron-interneuron synapse
Submillisecond AMPA receptor-mediated signaling at a principal neuron-interneuron synapse
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DOI：
10.1016/s0896-6273(00)80339-6
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发表时间：
1997-06-01
期刊：
影响因子：
16.2
通讯作者：
Jonas, P
中科院分区：
文献类型：
--
作者：
Geiger, JRP;Lubke, J;Jonas, P
通过在大鼠海马切片中的双重全细胞贴片钳记录与形态分析的双重全细胞贴片钳记录，研究了主要神经元间膜神经元突触的谷氨酸能传播。诱发的EPSP具有快速的时间过程（一半持续到大约4 ms； 34度C）是在靠近SOMA的间神经元树突上建立的多个突触接触处产生的。由于谷氨酸释放的精确时机和突触后AMPA受体的快速失活，突触后电导的基础变化显示出亚数的上升和衰减。基于中间神经元的A分室模型的模拟表明，迅速的突触后电导变化决定了EPSP的形状和体积整合，从而使中间神经元能够检测同步主神经元活性。
Glutamatergic transmission at a principal neuron-interneuron synapse was investigated by dual whole-cell patch-clamp recording in rat hippocampal slices combined with morphological analysis. Evoked EPSPs with rapid time course (half duration approximate to 4 ms; 34 degrees C) were generated at multiple synaptic contacts established on the interneuron dendrites close to the soma. The underlying postsynaptic conductance change showed a submillisecond rise and decay, due to the precise timing of glutamate release and the rapid deactivation of the postsynaptic AMPA receptors. Simulations based an a compartmental model of the interneuron indicated that the rapid postsynaptic conductance change determines the shape and the somatodendritic integration of EPSPs, thus enabling interneurons to detect synchronous principal neuron activity.