Spatial and temporal analysis of PCP protein dynamics during neural tube closure
Spatial and temporal analysis of PCP protein dynamics during neural tube closure
复制标题
DOI：
10.7554/elife.36456
复制
发表时间：
2018-08-06
期刊：
影响因子：
7.7
通讯作者：
Wallingford, John B.
中科院分区：
文献类型：
--
作者：
Butler, Mitchell T.;Wallingford, John B.
平面细胞极性（PCP）控制所有脊椎动物的会聚延伸和轴的伸长。虽然PCP蛋白的不对称定位是其功能的核心，但我们对会聚延伸过程中PCP蛋白的定位知之甚少。在这里，我们使用定量实时成像，同时监测非洲爪蟾神经板上皮细胞嵌入行为和PCP蛋白动力学。我们观察到PCP蛋白的不对称富集，但更有趣的是，我们观察到PCP蛋白富集与肌动球蛋白驱动的细胞-细胞连接的收缩行为密切相关。此外，我们发现连接PCP蛋白的周转率也与单个连接的收缩行为相关。当PCP信号被操纵时，所有这些动态关系都被破坏。总之，这些结果提供了一个动态和定量的视图，PCP蛋白定位在收敛延伸，并提出了一个复杂的和密切的联系之间的动态定位的核心PCP蛋白，肌动球蛋白组装，并在细胞嵌入在封闭的脊椎动物神经管的极化连接收缩。
Planar cell polarity (PCP) controls convergent extension and axis elongation in all vertebrates. Although asymmetric localization of PCP proteins is central to their function, we understand little about PCP protein localization during convergent extension. Here, we use quantitative live imaging to simultaneously monitor cell intercalation behaviors and PCP protein dynamics in the Xenopus laevis neural plate epithelium. We observed asymmetric enrichment of PCP proteins, but more interestingly, we observed tight correlation of PCP protein enrichment with actomyosin-driven contractile behavior of cell-cell junctions. Moreover, we found that the turnover rates of junctional PCP proteins also correlated with the contractile behavior of individual junctions. All these dynamic relationships were disrupted when PCP signaling was manipulated. Together, these results provide a dynamic and quantitative view of PCP protein localization during convergent extension and suggest a complex and intimate link between the dynamic localization of core PCP proteins, actomyosin assembly, and polarized junction shrinking during cell intercalation in the closing vertebrate neural tube.