STUDIES ON MECHANISM OF ACTION OF OXYGEN-LABILE HEMOLYSINS
STUDIES ON MECHANISM OF ACTION OF OXYGEN-LABILE HEMOLYSINS
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DOI：
10.1099/00221287-101-2-237
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
AULTMAN, KS
中科院分区：
文献类型：
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作者：
JOHNSON, MK;AULTMAN, KS
研究了溶血素溶血的结合步骤和溶血步骤对各种抑制剂的敏感性以及对测定条件的变化。HgCl2和n -乙基马来酰亚胺抑制其结合。先前固定的溶酶裂解对HgCl2不敏感，对n -乙基马来酰亚胺仅轻微敏感。溶血素的结合与离子强度无关。溶血素和溶血素O的结合分别在pH 8.0和8.4以上降低。结合可能需要一个非电离的未取代的巯基。用NaF孵育[兔]红细胞可抑制肺溶解，表明细胞的某些代谢功能可能参与了肺溶解。溶血素O的作用不受NaF的影响。
The sensitivities of the binding step and the lytic step of hemolysis by pneumolysin to the action of various inhibitors and to variations in the assay conditions were studied. Binding was inhibited by HgCl2 and N-ethylmaleimide. Lysis by previously fixed lysin was insensitive to HgCl2 and only slightly sensitive to N-ethylmaleimide. Binding of pneumolysin was independent of ionic strength. Binding of pneumolysin and streptolysin O decrease above pH 8.0 and 8.4, respectively. Binding may require a non-ionized unsubstituted sulfhydryl group. Incubation of [rabbit] erythrocytes with NaF caused inhibition of pneumolysis, indicating that some metabolic function of the cell may be involved in lysis. The action of streptolysin O was not affected by NaF.