Isolation and characterization of the human gene homologous to the Drosophila headcase (hdc) gene in chromosome bands 6q23-q24, a region of common deletion in human pancreatic cancer
Isolation and characterization of the human gene homologous to the Drosophila headcase (hdc) gene in chromosome bands 6q23-q24, a region of common deletion in human pancreatic cancer
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DOI：
10.3109/10425170109041340
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发表时间：
2001-01-01
期刊：
影响因子：
--
通讯作者：
Horii, A
中科院分区：
文献类型：
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作者：
Makino, N;Yamato, T;Horii, A
6号染色体长臂（6 q）缺失是多种人类恶性肿瘤中最常见的染色体异常之一。在此之前，我们已经确定了三个常见的缺失区域6 q（6 q21，6 q23-q24，和6 q26）在胰腺癌的杂合性丢失的研究，表明存在一个或多个肿瘤抑制基因在这个染色体arm. Using数据库搜索和cDNA文库筛选相结合，我们成功地从6 q24中分离出一个转录本。该mRNA编码由543个氨基酸组成的蛋白质，其与果蝇头壳（hdc）基因具有同源性，因此被命名为hHDC。北方分析鉴定了一个普遍表达的5.6 kb转录本。21个胰腺癌细胞系中的17个（81%）和5个肾细胞癌细胞系中的4个（80%）显示低水平表达，表明hHDC基因可能在某些人类癌症中起重要作用。
Deletions of the long arm of chromosome 6 (6q) are one of the most common chromosomal abnormalities in multiple human malignancies. Previously, we have identified three commonly deleted regions on 6q (6q21, 6q23-q24, and 6q26) in pancreatic cancer by loss of heterozygosity studies, suggesting the presence of one or more tumor suppressor genes on this chromosome arm. Using a combination of database search and cDNA library screening, we successfully isolated a transcript from 6q24. This mRNA encodes a protein consisting of 543 amino acids with homology to the Drosophila headcase (hdc) gene and, thus, is designated as hHDC. Northern analysis identified a ubiquitously expressed 5.6-kb transcript. Seventeen (81%) of 21 pancreatic cancer cell lines and four (80%) of five renal cell carcinoma cell lines showed low level expression, suggesting that the hHDC gene may play an important role in some human cancers.