NF-κB activation in human dental pulp stem cells by TNF and LPS
NF-κB activation in human dental pulp stem cells by TNF and LPS
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DOI：
10.1177/154405910508401105
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发表时间：
2005-11-01
影响因子：
7.6
通讯作者：
Wang, CY
中科院分区：
文献类型：
--
作者：
Chang, J;Zhang, C;Wang, CY
出生后的人牙髓干细胞（DPSC）代表牙髓中独特的前体细胞群，具有多潜能，可以再生牙本质/牙髓样结构。由于蛀牙导致牙髓经常受到口腔细菌的感染，因此在本研究中，我们检测了脂多糖（LPS）和肿瘤坏死因子（TNF）是否激活DPSC中的免疫转录因子核因子κB（NF-κB）。我们发现TNF和LPS均激活DPSC中的I-κB激酶复合物（IKK），诱导IκBα磷酸化和降解，导致NF-κB核转位。一致地，TNF和LPS均快速诱导NF-κB依赖性基因白细胞介素8（IL-8）的表达。然而，与单核细胞不同，我们发现LPS不能诱导DPSCs中NF-κB活性亚基p65的磷酸化。总之，我们的研究表明 DPSC 可能通过激活 NF-kappa B 参与牙髓感染期间的免疫反应。
Post-natal human dental pulp stem cells (DPSCs) represent a unique precursor population in the dental pulp, which has multipotential and can regenerate a dentin/pulp-like structure. Because the dental pulp is frequently infected by oral bacteria due to dental decay, in this study, we examined whether lipopolysaccharide (LPS) and tumor necrosis factor (TNF) activated the immunologic transcription factor nuclear factor kappa B (NF-kappa B) in DPSCs. We found that both TNF and LPS activated the I-kappa B kinase complex (IKK) in DPSCs to induce the phosphorylation and degradation Of I kappa B alpha, resulting in the nuclear translocation of NF-kappa B. Consistently, both TNF and LPS rapidly induced the expression of the NF-kappa B-dependent gene interteukin-8 (IL-8). However, unlike in monocytes, we found that LPS could not induce the phosphorylation of the NF-kappa B active subunit p65 in DPSCs. In summary, our studies suggest that DPSCs may be involved in immune responses during pulpal infection through activating NF-kappa B.