A polyubiquitin gene from Trypanosoma brucei.
A polyubiquitin gene from Trypanosoma brucei.
复制标题
来自布氏锥虫的多聚泛素基因。
DOI：
10.1016/0166-6851(89)90111-4
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发表时间：
1989
影响因子：
1.5
通讯作者：
Campbell,DA
中科院分区：
文献类型：
--
作者：
Wong,S;Campbell,DA
在低严格性下使用小鼠泛素克隆来鉴定T。来自粘粒文库（构建在载体c2×B中）的布鲁氏菌泛素序列。从阳性重组粘粒克隆（pJL4）中克隆了泛素杂交的228bpSalI片段（pUS02）。pUS02插入片段的全序列分析表明，它与小鼠泛素具有80%的同一性和94.7%的氨基酸同一性，证实其被选择为泛素基因。放射性标记的克隆T.布鲁氏菌泛素DNA与T.布氏杆菌基因组DNA显示4个区域序列相同。最强烈的杂交基因组带的一级结构。它含有多达30个泛素编码重复序列。T的主要组织。布鲁氏菌多聚泛素基因类似于酵母和其他生物，而不是相关的T。在布氏结核杆菌中，多聚泛素羧基末端是单个非泛素氨基酸延伸。
A mouse ubiquitin clone was used at low stringency to identifyT. brucei bruceiubiquitin sequences from a cosmid library (constructed in the vector c2×B). From a positive recombinant cosmid clone (pJL4) a ubiquitin-hybridizing 228bpSalI fragment (pUS02) was cloned. Complete sequencing of the pUS02 insert showed it to possess 80% identity and 94.7% amino acid identity with the mouse ubiquitin, confirming its selection as a ubiquitin gene. Hybridization of radiolabelled, clonedT. brucei bruceiubiquitin DNA to southern blots ofT. brucei bruceigenomic DNA revealed 4 regions of sequence identity. The primary structure of the most strongly hybridizing genomic band is presented. It contains up to 30 ubiquitin coding repeats. The primary organization of theT. brucei bruceipolyubiquitin gene resembles that of yeast and other organisms, rather than that of the relatedT. cruzi.InT. brucei bruceithe polyubiquitin carboxy terminus is a single, non-ubiquitin amino acid extension.