Temperature Pretreatment Alters the Polarization Response of Human Neutrophils to the Chemoattractant N-formyl-Met-Leu-Phe
Temperature Pretreatment Alters the Polarization Response of Human Neutrophils to the Chemoattractant N-formyl-Met-Leu-Phe
复制标题
DOI：
10.1007/s10753-008-9101-3
复制
发表时间：
2009-02
期刊：
影响因子：
5.1
通讯作者：
Dongliang Zhao;Xiaojing Meng;C. Cai;Chun-hua Yuan;Fei Zou
中科院分区：
文献类型：
--
作者：
Dongliang Zhao;Xiaojing Meng;C. Cai;Chun-hua Yuan;Fei Zou
中性粒细胞在化学引诱物的刺激下呈现极化形态，其在宿主防御机制中起重要作用。已经发表了许多关于中性粒细胞极化的研究，其中使用了三种不同的温度预处理（4°C、25°C和37°C）。然而，没有研究调查是否不同的温度预处理影响中性粒细胞极化。在目前的研究中，我们检查了4°C，25°C和37°C预处理温度对短期（1或3分钟）化学引诱剂诱导的极化的影响。人中性粒细胞经不同温度预处理后，在N-甲酰-蛋氨酸-亮氨酸-苯丙氨酸（fMLP）刺激下发生极化。光镜下观察中性粒细胞的形态学变化。用免疫组织化学法测定F-肌动蛋白聚合。在25°C和37°C预处理组中存在比4°C组（32.4%）更多的头-尾极化细胞（>50%的细胞）。4°C、25°C和37°C预处理组的伪足平均长度分别为3.2 ± 1.1 μ m（n= 17）、5.3 ± 2.1 μm（n= 12）和7.4 ± 2.7 μm（n= 21），4°C和37 ° C预处理组之间差异有统计学意义（P< 0.05）。此外，在用fMLP刺激的1分钟内，三组之间的伪足延伸率以及4°C组与其他两组之间的片足百分比存在统计学显著差异。这项研究表明，不同的温度预处理影响中性粒细胞极化后，短期刺激与fMLP。
Neutrophils present a polarized morphology upon stimulation of chemoattractants, which play a vital role in host-defense mechanisms. Many studies have been published on neutrophil polarization, in which three different temperatures pretreatment (4°C, 25°C and 37°C) have been used. However, no study has investigated whether different temperature pretreatments affect neutrophil polarization. In the current study, we examined the effects of 4°C, 25°C and 37°C pretreatment temperatures on short-term (1 or 3 min) chemoattractant-induced polarization. Human neutrophils were polarized upon the stimulation ofN-formyl-Met-Leu-Phe (fMLP) after pretreated by different temperature. The morphological changes of the neutrophils were investigated under the microscopy. The F-actin polymerization was determined by immunological histological chemistry. There were more head–tail polarized cells (>50% of the cells) in the 25°C and 37°C pretreatment groups than in the 4°C group (32.4%). The average lengths of the pseudopod were 3.2 ± 1.1 μm (n= 17), 5.3 ± 2.1 μm (n= 12) and 7.4 ± 2.7 μm (n= 21) in the 4°C, 25°C and 37°C pretreatment groups, respectively; the 4°C and 37°C pretreatment groups were statistically different (P< 0.05). Additionally, there was a statistically significant difference in the pseudopod extension rate among the three groups, as well as the Lamellipod percentage between the 4°C group and the other two groups within 1 min of stimulation with fMLP. This study demonstrates that different temperature pretreatments affect neutrophil polarization upon short-term stimulation with fMLP.