Influence of the duration of ischemia and reperfusion on infarct volume and microvascular damage in mice
Influence of the duration of ischemia and reperfusion on infarct volume and microvascular damage in mice
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DOI：
10.1179/016164105x48789
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发表时间：
2006-03
影响因子：
1.9
通讯作者：
M. Vosko;D. Burggraf;M. Liebetrau;N. Wunderlich;G. Jäger;M. Gröger;N. Plesnila;G. Hamann
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文献类型：
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作者：
M. Vosko;D. Burggraf;M. Liebetrau;N. Wunderlich;G. Jäger;M. Gröger;N. Plesnila;G. Hamann
摘要目标：局灶性脑缺血导致血管通透性改变，微血管完整性的丧失是随后脑缺血的主要原因。我们评估了小鼠局灶性脑缺血后不同缺血和再灌注时间对梗死面积和微血管损伤的影响。方法：C57 BL/6小鼠（n = 39）行局灶性脑缺血（I）再灌注（R）。分析连续脑切片的梗死体积（Nissl染色）和IV型胶原（免疫组织化学和蛋白质印迹）。结果如下：梗死面积（梗死体积相对于同侧半球的百分比）随缺血和再灌注总时间增加：19 ± 2%（I3 R 0），30 ± 2%（I3 R3），36 ± 4%（I3 R12），41 ± 4%（I1 R24），45 ± 6%（I2 R24）和58 ± 2%（I3 R24）。缺血半球显示IV型胶原阳性血管（缺血与非缺血对侧区域）显著进行性减少：90 ± 3%（I3 R 0）、88 ± 1%（I3 R3）、82 ± 3%（I3 R12）、85 ± 3%（I1 R24）、79 ± 3%（I2 R24）、72 ± 2%（I3 R24）。结论：长时间缺血和再灌注均可导致梗死体积增加，以及进行性微血管损伤。
Abstract Objectives: Focal cerebral ischemia is responsible for alterations of vascular permeability, and the loss of microvascular integrity is a primary source of subsequent hemorrhages. We evaluated the influence of different durations of ischemia and reperfusion on infarction size and microvascular damage after focal cerebral ischemia in the mouse. Methods: C57BL/6 mice (n = 39) were subjected to focal cerebral ischemia (I) and reperfusion (R). Consecutive brain sections were analysed for infarction volumes (Nissl-staining) and for collagen type IV (immunohistochemistry and western blot). Results: Infarction size (percentage of the infarction volume versus ipsilateral hemisphere) increased with total time of ischemia and reperfusion: 19 ± 2% (I3R0), 30 ± 2% (I3R3), 36 ± 4% (I3R12), 41 ± 4% (I1R24), 45 ± 6% (I2R24) and 58 ± 2% (I3R24). The ischemic hemispheres showed a significant progressive reduction of collagen type IV positive vessels (ischemic versus non-ischemic contralateral area): 90 ± 3% (I3R0), 88 ± 1% (I3R3), 82 ± 3% (I3R12), 85 ± 3% (I1R24), 79 ± 3% (I2R24), 72 ± 2% (I3R24). Conclusions: Both prolonged ischemia and reperfusion lead to an increased infarction volume, as well as progressive microvascular damage.