Ultrasonic quantitation of superficial degradation of articular cartilage
Ultrasonic quantitation of superficial degradation of articular cartilage
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DOI：
10.1016/j.ultrasmedbio.2004.03.005
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发表时间：
2004-06-01
影响因子：
2.9
通讯作者：
Jurvelin, JS
中科院分区：
文献类型：
--
作者：
Saarakkala, S;Töyräs, J;Jurvelin, JS
超声（US）已被建议作为定量检测关节软骨中早期骨关节病变化的一种手段。在这项研究中，定量US 2-D成像（20 MHz）的能力，揭示机械或酶降解后的牛关节软骨表面的变化进行了研究，在体外。机械降解是由研磨样品对金刚砂纸与粒度为250 μ m，106 μ m，45 μ m或23 μ m。对于酶促降解，用胶原酶、胰蛋白酶或软骨素酶ABC消化样品。研究了由降解引起的US反射系数的变化。此外，两个新的参数，美国粗糙度指数（URI）和美国反射系数（SVR）的空间变化，建立量化的软骨表面的完整性。在机械降解或胶原酶酶消化后，观察到US反射系数的统计学显著降低（p < 0.05）。在这些治疗后，URI也有所增加（p < 0.05）。我们的结论是，定量超声成像可用于检测关节面的胶原蛋白破坏和粗糙度增加。这些结构性损伤是早期骨关节病的典型表现。（电子邮件：saarakka@hytti.uku.fi）（C）2004年世界医学超声生物学联合会。
Ultrasound (US) has been suggested as a means for the quantitative detection of early osteoarthrotic changes in articular cartilage. In this study, the ability of quantitative US 2-D imaging (20 MHz) to reveal superficial changes in bovine articular cartilage after mechanical or enzymatic degradation was investigated in vitro. Mechanical degradation was induced by grinding samples against an emery paper with the grain size of 250 mum, 106 mum, 45 mum or 23 mum. For enzymatic degradation, samples were digested with collagenase, trypsin or chondroitinase ABC. Variations of the US reflection coefficient induced by the degradation were investigated. Furthermore, two novel parameters, the US roughness index (URI) and the spatial variation of the US reflection coefficient (SVR), were established to quantitate the integrity of the cartilage surface. Statistically significant decreases (p < 0.05) in US reflection coefficient were observed after mechanical degradations or enzymatic digestion with collagenase. Increases (p < 0.05) in URI were also revealed after these treatments. We conclude that quantitative US imaging may be used to detect collagen disruption and increased roughness in the articular surface. These structural damages are typical of early osteoarthrosis. (E-mail: saarakka@hytti.uku.fi) (C) 2004 World Federation for Ultrasound in Medicine Biology.