Noncomplementary DNA double-strand-break rejoining in bacterial and human cells.
Noncomplementary DNA double-strand-break rejoining in bacterial and human cells.
复制标题
细菌和人类细胞中非互补 DNA 双链断裂重新连接。
DOI：
10.1093/nar/21.5.1055
复制
发表时间：
1993
影响因子：
14.9
通讯作者：
Morgan,WF
中科院分区：
文献类型：
--
作者：
King,JS;Valcarcel,ER;Rufer,JT;Phillips,JW;Morgan,WF
We examinied the rejoining of noncomplementary restriction enzyme-produced DNA double-strand breaks inEscherichia coliand in cultured human cells. The enzymes used in this study, ClaI, BamHI and SalI, produce double-strabd breaks with 5 protruding single strands. The joining of a Clal-produced DNA end to a BamHI-produced end or to a SalI-produced end was examined at the DNA sequence level. End rejoining inE.Coliwas studied by transforming cultures with linear plasmid DNA that was gel purified from restriction digests, and end rejoining in cultured human cells was studies by introducing enzymes into the cells by electroporation. The human cells used contain an Epstein-Barr virus (EBV)-based shuttle vecotr, pHAZE, that was recovered and introduced intoE.colifor further analysis. The major products of Dna end-joining processes observed in linear plasmid-transformedE.coliand in the human cells exposed to restriction enzymes were identical. Furthermore, the deletions observed in both systems and in the spontaneous mutant plasmids in untreated human cells had a common underlying feature: short stretches of directly repeated DNA at the junction sites.
