IRIDOVIRUS INFECTION OF CULTURED RED-SEA BREAM, PAGRUS-MAJOR
IRIDOVIRUS INFECTION OF CULTURED RED-SEA BREAM, PAGRUS-MAJOR
复制标题
DOI：
10.3147/jsfp.27.19
复制
发表时间：
1992-03-01
期刊：
影响因子：
0.6
通讯作者：
SORIMACHI, M
中科院分区：
文献类型：
--
作者：
INOUYE, K;YAMANO, K;SORIMACHI, M
1990年，在四国岛上发生了一次新的流行性流行病。患病鱼游泳不活跃，表现为严重贫血，死亡率为20%-60%。在脾、心、肾、肝和鳃中观察到典型的以嗜血染色为特征的增大细胞。从这些细胞的形态和定位来看，它们被认为是白细胞。在这些细胞的细胞质中可见六角形病毒粒子。每个病毒粒子由一个中心电子致密核(120 Nm)和一个电子半透明带组成，直径为200-240 nm。扩大的细胞的Feulgen染色表明病毒中有DNA。这些结果表明该病毒属于虹彩病毒科。病毒在20-25℃时缓慢复制，并在RTG-2、CHSE-214、FHM、BF-2和KRE-3细胞中产生扩大和圆形的细胞病变效应。将感染鱼的脾匀浆滤液(450 Nm)经腹膜腔内接种至罗非鱼鱼种，可引起与自然发病鱼相似的病理变化。
A new epizootic occurred in red sea bream, Pagrus major, in Shikoku Island in 1990. Diseased fish swam inactively and showed severe anemia with 20-60% mortality. Typically enlarged cells characterized by bathophilic stainability were observed in the spleen, heart, kidney, liver and gill. From the morphology and localization of these cells, they are considered leucocytes. Hexagonal virions were found in the cytoplasm of these cells. Each virion consisted of a central electron-dense core (120 nm) and an electron translucent zone and measured 200-240 nm in diameter. Feulgen staining of the enlarged cells suggested DNA in the virus. These results suggest that the virus belongs to the family Iridoviridae. The virus slowly replicated and produced cytopathic effects of enlarged and rounded cells in RTG-2, CHSE-214, FHM, BF-2 and KRE-3 cells at 20-25-degrees-C. Intraperitoneal inoculation of the filtrate (450 nm) of spleen homogenate of the infected fish to red sea bream fingerlings induced similar pathological changes to those observed in spontaneously diseased fish.