Pitfalls in TCR gene clonality testing: teaching cases.
Pitfalls in TCR gene clonality testing: teaching cases.
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DOI：
10.1007/s12308-008-0013-9
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发表时间：
2008-09-01
影响因子：
0.6
通讯作者：
van Krieken, Johan H J M
中科院分区：
文献类型：
--
作者：
Groenen, Patricia J T A;Langerak, Anton W;van Krieken, Johan H J M
T淋巴细胞增殖中的克隆性检测在技术上已经变得相对容易在常规实验室中使用由BIOMED-2 Concerted Action BMH 4-CT 98 -3936开发的用于T细胞受体（TCR）基因分析的标准化多重聚合酶链反应方案进行。克隆性诊断的专业知识和TCR基因重组的生物学知识对于正确解释TCR克隆性数据至关重要。本报告讨论了应考虑的几个免疫生物学和技术陷阱，以避免对数据的误解。此外，我们讨论了需要整合的分子数据与那些从免疫组织学，最好也流式细胞术免疫表型，适当的解释。这种交互式的多学科诊断模型保证了现有数据的整合，以达到最可靠的诊断。
Clonality testing in T-lymphoproliferations has technically become relatively easy to perform in routine laboratories using standardized multiplex polymerase chain reaction protocols for T-cell receptor (TCR) gene analysis as developed by the BIOMED-2 Concerted Action BMH4-CT98-3936. Expertise with clonality diagnostics and knowledge about the biology of TCR gene recombination are essential for correct interpretation of TCR clonality data. Several immunobiological and technical pitfalls that should be taken into account to avoid misinterpretation of data are addressed in this report. Furthermore, we discuss the need to integrate the molecular data with those from immunohistology, and preferably also flow cytometric immunophenotyping, for appropriate interpretation. Such an interactive, multidisciplinary diagnostic model guarantees integration of available data to reach the most reliable diagnosis.