Detection of two alternative bcr/abl mRNA junctions and minimal residual disease in Philadelphia chromosome positive chronic myelogenous leukemia by polymerase chain reaction.
Detection of two alternative bcr/abl mRNA junctions and minimal residual disease in Philadelphia chromosome positive chronic myelogenous leukemia by polymerase chain reaction.
复制标题
通过聚合酶链反应检测费城染色体阳性慢性粒细胞白血病中的两种替代 bcr/abl mRNA 连接和微小残留病。
作者：
Ming;A. Lemaistre;H. Kantarjian;Moshe Talpaz;Emil J. Freireich;Jose M. Trujillo;Sanford A. Stass
慢性粒细胞白血病（CML）中的费城（Ph '）染色体导致bcr基因和c-abl癌基因融合，其转录成两种类型的嵌合bcr/abl mRNA：L-6连接和K-28连接。应用逆转录-聚合酶链反应（RT/PCR）技术，对31例Ph ′阳性CML和2例Ph ′阴性bcr重排CML患者的38份血标本进行了分析。在慢性期获得的21个样本中，8个患者具有L-6 mRNA，11个具有K-28 mRNA，2个具有L-6和K-28 mRNA。在胚泡危象中获得的9个样品中，4个含有L-6 mRNA，2个含有K-28 mRNA，3个含有K-28和L-6 mRNA。这一发现支持Ph '阳性CML中转录的bcr/abl mRNA的选择性剪接的概念。然而，这似乎是一个罕见的事件。从8例经重组α-干扰素（r α-IFN）治疗6个月至3年后细胞遗传学完全缓解和bcr区重排阴性的患者中获得的8份样本中，所有样本均显示出最小残留Ph '阳性克隆的证据。这一发现表明干扰素治疗抑制了Ph ′阳性克隆的增殖，但不能完全根除Ph ′阳性干细胞。
The Philadelphia (Ph') chromosome in chronic myelogenous leukemia (CML) results in fusion of the bcr gene and c-abl oncogene, which transcribes into two types of chimeric bcr/abl mRNAs: the L-6 junction and the K-28 junction. By means of a highly sensitive assay, combination of reverse transcription and polymerase chain reaction (RT/PCR), we analyzed 38 blood samples obtained from 31 patients with Ph'-positive CML and two patients with Ph'-negative bcr rearranged CML. Among the 21 samples obtained in chronic phase, eight patients had the L-6 mRNA, 11 had the K-28 mRNA, and two had both the L-6 and K-28 mRNAs. Among the nine samples obtained in blast crisis, four contained the L-6 mRNA, two contained the K-28 mRNA, and three contained both the K-28 and L-6 mRNAs. This finding supports the concept of alternative splicing of bcr/abl mRNAs transcribed in Ph'-positive CML. However, it appears to be a rare event. Of the eight samples obtained from eight patients who had achieved complete cytogenetic remission and negativity for bcr region rearrangement for 6 months to 3 years after recombinant alpha interferon (r alpha-IFN) therapy, all of them showed evidence of minimal residual Ph'-positive clones as detected by the RT/PCR assay. This finding suggests that interferon therapy suppresses the proliferation of the Ph'-positive clones, but it does not completely eradicate the Ph'-positive stem cells.
慢性粒细胞白血病患者的 bcr-abl RNA。
影响因子：
20.3
作者：
Shtivelman,E;Gale,RP;Dreazen,O;Berrebi,A;Zaizov,R;Kubonishi,I;Miyoshi,I;Canaani,E
通讯作者：
Canaani,E