Single-cell RNA sequencing to explore composition of peripheral blood NK cells in patients with chronic myeloid leukemia in treatment-free remission
Single-cell RNA sequencing to explore composition of peripheral blood NK cells in patients with chronic myeloid leukemia in treatment-free remission
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单细胞 RNA 测序探讨慢性粒细胞白血病免治疗缓解期患者外周血 NK 细胞的组成
DOI：
10.1080/10428194.2022.2086243
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发表时间：
2022-06-10
影响因子：
2.6
通讯作者：
Xu，Dan
中科院分区：
文献类型：
--
作者：
Yu，Guopan;Lu，Weixiang;Xu，Dan
摘要本研究旨在探讨NK细胞亚群和基因表达在维持TFR状态中的作用。我们在两组(健康对照组和TFR患者)的PBMCs中鉴定出六种类型的NK细胞。基因肿瘤学分析表明，上调的基因在“免疫反应”、“对肿瘤细胞的反应”和“细胞溶解”等类别中得到丰富。此外，我们还发现CML-TFR患者外周血中成熟和终末期NK细胞、CD56明亮NK细胞和过渡性NK细胞三个亚群均含有许多显著上调的基因，CD56明亮NK细胞和过渡性NK细胞处于增殖和激活状态。通过单细胞RNA测序分析，我们证实NK细胞的成熟亚群、终末亚群、CD56亚群和过渡性亚群在维持CML患者的TFR中起着不可或缺的作用。
Abstract This study was to explore the role of NK cell subsets and gene expression in maintaining TFR status. We identified six types of NK cells in the PBMCs over both groups (healthy controls and patients with TFR). Gene Oncology analysis showed that up regulated genes were enriched in the categories of “immune response,” “reaction to tumor cells,” and “cytolysis.” In addition, we found that the three NK cell subsets, mature and terminal NK cells, CD56 bright NK cells, and transitional NK cells, contained many significantly up regulated genes in both groups, and that CD56 bright NK cells and transitional NK cells in patients with CML-TFR were in a proliferating and activated state. Through single-cell RNA sequencing analysis, we confirmed that the mature and terminal, CD56 bright, and transitional subsets of NK cells play an indispensable role in maintaining TFR in patients with CML.