Characterization of Leuconostoc gasicomitatum sp nov., associated with spoiled raw tomato-marinated broiler meat strips packaged under modified-atmosphere conditions
Characterization of Leuconostoc gasicomitatum sp nov., associated with spoiled raw tomato-marinated broiler meat strips packaged under modified-atmosphere conditions
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DOI：
10.1128/aem.66.9.3764-3772.2000
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发表时间：
2000-09-01
影响因子：
4.4
通讯作者：
Vandamme, P
中科院分区：
文献类型：
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作者：
Björkroth, KJ;Geisen, R;Vandamme, P
根据含有编码16S和23S rRNAs(RDNA)的限制性片段长度多态性(RFLP)(核糖体分型)数据库，鉴定了与气调包装、生番茄腌制的肉条气态腐败有关的乳酸菌(LAB)。混合实验室种群由一种类似于明串珠菌的种主导，导致了产品的变质。检测到的其他主要微生物是萨凯乳杆菌、弯曲乳杆菌和一株革兰氏阳性杆菌，其表型类似于异源发酵乳杆菌。PH值的升高和包装的极度膨胀表明了一种罕见的实验室腐败类型，称为“蛋白质膨胀”。这种腐败的特征是由于氨基酸脱羧基产生过量的气体，而pH的升高归因于随后的氨基酸脱氨基。此前，蛋白质膨胀与任何一种肉类产品都没有关联。采用经典表型、全细胞蛋白质电泳、16和23S rDNA RFLP、16S rDNA序列分析和DNA-DNA重结合分析等多相方法对明珠藻属的优势种进行了鉴定，并进行了RFLP分析、表型分析、全细胞蛋白质分析和16S rDNA序列同源性分析，结果表明，石蒜与腐败相关种的相似性最高。两株腐败菌株的16S rDNA序列与Gelium型菌株的同源性分别为98.8%和99.0%，DNA-DNA重排明显区分了这两个种。同样的菌株与灵芝类型菌株的杂交仅有22%和34%。这些结果需要一个单独的物种地位，我建议将其命名为Leuconostoc gasicomitata sp.11月对于这种腐败相关的亮绿藻物种。
Lactic acid bacteria (LAB) associated with gaseous spoilage of modified-atmosphere-packaged, raw, tomato-marinated broiler meat strips were identified on the basis of a restriction fragment length polymorphism (RFLP) (ribotyping) database containing DNAs coding for 16S and 23S rRNAs (rDNAs). A mixed LAB population dominated by a Leuconostoc species resembling Leuconostoc gelidum caused the spoilage of the product. Lactobacillus sakei, Lactobacillus curvatus, and a gram-positive rod phenotypically similar to heterofermentative Lactobacillus species were the other main organisms detected. An increase in pH together with the extreme bulging of packages suggested a rare LAB spoilage type called "protein swell." This spoilage is characterized by excessive production of gas due to amino acid decarboxylation, and the rise in pH is attributed to the subsequent deamination of amino acids. Protein swell has not previously been associated with any kind of meat product. A polyphasic approach, including classical phenotyping, whole-cell protein electrophoresis, 16 and 23S rDNA RFLP, 16S rDNA sequence analysis, and DNA-DNA reassociation analysis, was used for the identification of the dominant Leuconostoc species, in addition to the RFLP analysis, phenotyping, whole-cell protein analysis, and 16S rDNA sequence homology indicated that L. gelidum was most similar to the spoilage-associated species. The two spoilage strains studied possessed 98.8 and 99.0% 16S rDNA sequence homolog with the L. gelidum type strain, DNA-DNA reassociation, however, clearly distinguished the two species. The same strains showed only 22 and 34% hybridization with the L. gelidum type strain. These results warrant a separate species status, and me propose the name Leuconostoc gasicomitatum sp. nov. for this spoilage-associated Leuconostoc species.