Expression of aquaporin-1 in human peritoneal mesothelial cells and its upregulation by glucose in vitro
Expression of aquaporin-1 in human peritoneal mesothelial cells and its upregulation by glucose in vitro
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DOI：
10.1681/asn.v1251036
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发表时间：
2001-05-01
影响因子：
13.6
通讯作者：
Leung, JC
中科院分区：
文献类型：
--
作者：
Lai, KN;Li, FK;Leung, JC
水通道蛋白 (AQP) 是一个水通道家族，对水（偶尔还有甘油）的通过具有高度选择性。在之前的研究中，在对照受试者和腹膜透析患者的人腹膜内皮细胞中仅发现了AQP1。由于人腹膜间皮细胞（HPMC）在连续不卧床腹膜透析患者的透析充分性和液体平衡中发挥着重要作用，本研究检测了HPMC中是否存在AQP1，发现AQP1 mRNA和蛋白组成型地存在于HPMC中。通过人腹膜间皮衬里的双重免疫组织化学染色证实了 AQP1 蛋白在腹膜间皮细胞中的定位。更重要的是，HPMC 中 AQP1 的表达不是组成型的，HPMC 中 AQP1 的转录和生物合成是由葡萄糖和甘露醇等渗透剂诱导的。暴露于葡萄糖后，AQP1 生物合成显着增强，且呈时间和剂量依赖性（P < 0.0001）。在内皮细胞系 EA.hy 926 的 AQP1 生物合成中观察到类似的结果。特别有趣的是，当在相同设置下进行实验时，间皮细胞中 AQP1 mRNA 或生物合成的上调始终显着高于内皮细胞 (P < 0.001)。首次证明 HPMC 中 AQP1 的表达。渗透剂上调该水通道蛋白的 mRNA 和蛋白质表达。HPMC 中 AQP1 在维持腹膜超滤中的作用可能具有临床意义。
Aquaporin (AQP) is a family of water channels that are highly selective for the passage of water and occasionally glycerol. In previous studies, only AQP1 was found in human peritoneal endothelial cells in both control subjects and patients on peritoneal dialysis. As human peritoneal mesothelial cells (HPMC) play an important role in dialysis adequacy and fluid balance in continuous ambulatory peritoneal dialysis patients, this study examined whether AQP1 is present in HPMC, It was found that AQP1 mRNA and protein are present in HPMC constitutively. The localization of AQP1 protein in peritoneal mesothelial cells was confirmed by double immunohistochemical staining of the mesothelial lining of human peritoneal membrane. More important, the expression of AQP1 in HPMC is not constitutive and the transcription and biosynthesis of AQP1 in HPMC is inducible by osmotic agents such as glucose and mannitol, There was significant enhancement of AQP1 biosynthesis upon exposure to glucose in a time- and dose-dependent manner (P < 0.0001). Similar findings were observed in the AQP1 biosynthesis by an endothelial cell line, EA.hy 926. Of particular interest, the upregulation in AQP1 mRNA or biosynthesis in mesothelial cells was always significantly higher than that of endothelial cells when the experiments were conducted under identical settings (P < 0.001). AQP1 expression in HPMC was demonstrated for the first time. Osmotic agents upregulate both mRNA and protein expression of this aquaporin, The role of AQP1 in HPMC in maintaining the ultrafiltration of the peritoneal membrane is potentially of clinical interest.