TAP-independent, beta 2-microglobulin-dependent surface expression of functional mouse CD1.1.
TAP-independent, beta 2-microglobulin-dependent surface expression of functional mouse CD1.1.
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DOI：
10.1084/jem.182.6.1913
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发表时间：
1995-12-01
期刊：
影响因子：
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通讯作者：
Bendelac A
中科院分区：
文献类型：
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作者：
Brutkiewicz RR;Bennink JR;Yewdell JW;Bendelac A
CD 1分子由β 2-微球蛋白（β 2 m）与非主要组织相容性复合体（MHC）编码的单态整合膜蛋白（与MHC I类α链同源）非共价复合组成。关于CD 1的细胞表面表达和T细胞识别的要求知之甚少。我们将小鼠CD 1.1基因插入到牛痘病毒中，以产生在病毒启动子控制下表达CD 1.1的重组病毒。使用这种重组病毒感染正常或突变细胞系，我们发现，与CD1.1特异性单克隆抗体3C 11反应的分子的表达需要β 2 m的表达，但不受MHC编码的肽转运蛋白（TAP）的情况下。与这些结果一致，正常小鼠或TAP 1基因纯合缺失小鼠的胸腺细胞诱导mCD 1.1特异性T细胞杂交瘤DN32.D3产生IL-2，但β 2 m基因纯合缺失小鼠的胸腺细胞不诱导。这些结果表明功能性mCD1.1的表达以β 2 m依赖性、TAP非依赖性的方式发生。
CD1 molecules consist of beta 2-microglobulin (beta 2m) noncovalently complexed to a non-major histocompatibility complex (MHC)-encoded monomorphic integral membrane protein homologous to MHC class I alpha chains. Little is known about the requirements for cell surface expression and T cell recognition of CD1. We inserted the mouse CD1.1 gene into vaccinia virus to create a recombinant virus expressing CD1.1 under the control of a viral promoter. Using this recombinant virus to infect normal or mutant cell lines, we found that the expression of molecules reactive with the CD1.1-specific monoclonal antibody 3C11 requires the expression of beta 2m but was not affected by the absence of the MHC-encoded peptide transporter (TAP). Consistent with these results, IL-2 production by the mCD1.1-specific T cell hybridoma DN32.D3 was induced by thymocytes from normal mice or mice with a homozygous deletion of the TAP1 gene, but not by thymocytes from mice with a homozygous deletion of the beta 2m gene. These results indicate that expression of functional mCD1.1 occurs in a beta 2m-dependent, TAP- independent manner.