Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder (Paralichthys olivaceus)
Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder (Paralichthys olivaceus)
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DOI：
10.1016/j.aquaculture.2007.07.210
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发表时间：
2007-11-26
期刊：
影响因子：
4.5
通讯作者：
Okamoto, Nobuaki
中科院分区：
文献类型：
--
作者：
Fuji, Kanako;Hasegawa, Osamu;Okamoto, Nobuaki
微卫星Poli9-8TUF等位基因在单个主要位点具有显性作用，并负责日本比牙鲆对淋巴囊肿病（LD-R）的抗性。利用该等位基因进行标记辅助育种，开发了一种新的比目鱼种群。亲本选择来自于有利等位基因（b -有利）纯合子的lp - b自交系的雌性和来自于生长速度高、体型好的商业砧木的雄性。选择雌性作为携带LD-R的亲本，是因为在LD-R位点所在的区域，雌性的重组率较低。正如预期的那样，b有利等位基因作为杂合子传递给后代（LD-R+群体）。当在两个发生LD暴发的商业养鱼场对LD- r +种群进行测试时，两个养殖场都没有LD发病率，而没有b有利等位基因（LD- r -）的对照种群在两个养殖场的发病率分别为4.5%和6.3%。这些结果表明，利用与重要经济性状相关的分子标记进行标记辅助育种是一种有效的育种策略。(c) 2007 Elsevier B.V.版权所有
An allele of a microsatellite, Poli9-8TUF, has a dominant effect at a single major locus and is responsible for resistance to lymphocystis disease (LD-R) in Japanese flounder. We developed a new population of Japanese flounder produced by marker-assisted breeding using this allele. A female that originated from the KP-B inbred line with LD-R that was homozygous for the favorable allele (B-favorable) and a male from a commercial stock bred for higher growth rate and good body shape were selected as parents. A female was selected as the LD-R-bearing parent because the recombination rate of females is lower in the region where the LD-R locus is located. As expected, the B-favorable allele was transmitted as a heterozygote to the progeny (LD-R+ population). The LD-R+ population, when tested at two commercial fish farms that had LD outbreaks, showed no incidence of LD at either farm, while a control population without B-favorable alleles (LD-R-) had incidences of 4.5% and 6.3% at the two farms. These results show that marker-assisted breeding using molecular markers linked to an economically important trait is an efficient strategy for breeding. (c) 2007 Elsevier B.V. All rights reserved.