Recombinant fragment of protein kinase inhibitor blocks cyclic AMP-dependent gene transcription.
Recombinant fragment of protein kinase inhibitor blocks cyclic AMP-dependent gene transcription.
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蛋白激酶抑制剂的重组片段可阻断环 AMP 依赖性基因转录。
DOI：
10.1126/science.2821622
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发表时间：
1987
期刊：
影响因子：
56.9
通讯作者：
J. Avruch
中科院分区：
文献类型：
--
作者：
Grove;D. Price;H. Goodman;J. Avruch
哺乳动物细胞中环磷酸腺苷 (cAMP) 的转录调节可以由 cAMP 依赖性蛋白激酶的磷蛋白底物介导，或者如在原核生物中，由 cAMP 结合蛋白介导。构建了编码该激酶的蛋白质抑制剂的活性片段和突变体非活性片段的两个合成基因，并用于区分这些替代方案。活性肽产物的瞬时表达特异性抑制共转染报告基因的cAMP刺激表达超过90%，而无活性肽的表达不会改变cAMP刺激的基因表达。结果表明，活性激酶催化亚基是 cAMP 刺激基因转录的必要中间体。
Transcriptional regulation by cyclic adenosine monophosphate (cAMP) in mammalian cells could be mediated by a phosphoprotein substrate of the cAMP-dependent protein kinase or, as in prokaryotes, by a cAMP-binding protein. Two synthetic genes that code for an active fragment of the protein inhibitor of this kinase and a mutant inactive fragment were constructed and used to distinguish these alternatives. Transient expression of the active peptide product specifically inhibited the cAMP-stimulated expression of a cotransfected reporter gene by more than 90 percent, whereas the expression of the inactive peptide did not alter cAMP-stimulated gene expression. The results indicate that an active kinase catalytic subunit is a necessary intermediate in the cAMP stimulation of gene transcription.
DOI：
10.1073/pnas.83.18.6682
发表时间：
1986-09-01
影响因子：
11.1
作者：
MONTMINY, MR;SEVARINO, KA;GOODMAN, RH
通讯作者：
GOODMAN, RH
来自兔骨骼肌的 cAMP 依赖性蛋白激酶热稳定性抑制剂的氨基酸序列。
DOI：
10.1073/pnas.82.17.5732
发表时间：
1985
影响因子：
11.1
作者：
Scott,JD;Fischer,EH;Takio,K;Demaille,JG;Krebs,EG
通讯作者：
Krebs,EG